Real-time detection of cardiac troponin I and mechanism analysis of AlGaAs/GaAs high electron mobility transistor biosensor

The high electron mobility transistor (HEMT)-based biosensors are highly competitive in the ultimate application of portable and point-of-care testing. Herein, we have demonstrated highly sensitive and real-time detection of cardiac troponin I (cTnI), a biomarker for the diagnosis of acute myocardia...

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Bibliographic Details
Published in:AIP advances 2020-11, Vol.10 (11), p.115205-115205-7
Main Authors: Luo, Jiaming, Li, Sufang, Xu, Mengke, Guan, Min, Yang, Mengxi, Ren, Jingyi, Zhang, Yang, Zeng, Yiping
Format: Article
Language:English
Subjects:
Aluminum gallium arsenides
Antibodies
Biomarkers
Biosensors
Buffer solutions
Diagnosis
High electron mobility transistors
Myocardial infarction
Real time
Response time
Semiconductor devices
Transistors
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Summary:The high electron mobility transistor (HEMT)-based biosensors are highly competitive in the ultimate application of portable and point-of-care testing. Herein, we have demonstrated highly sensitive and real-time detection of cardiac troponin I (cTnI), a biomarker for the diagnosis of acute myocardial infarction (AMI) using AlGaAs/GaAs HEMT-based biosensors. The device has achieved a lower detection limit of 1 pg/ml in the buffer solution and less than 30 s response time, which demonstrated significant promise in the early diagnosis and screening of AMI. In addition, our results are consistent with the enzyme-linked immunosorbent assay according to the AMI patient’s blood test results. Furthermore, by comparing the two HEMT structures, we also calculated the equilibrium dissociation constant (KD) of the cTnI and cTnI antibody and analyzed the sensing mechanism. The results show that this method is very promising for early diagnosis of AMI.
ISSN:2158-3226
2158-3226
DOI:10.1063/5.0027553