Highly efficient biocatalytic cascade for the diversity-oriented synthesis of complex blood group Sda antigens

Sda antigens are a family of widely distributed histo-blood group antigens sharing the Siaα2,3(GalNAcβ1,4)Gal trisaccharide determinant. Despite their significant biological importance, the synthesis of these complex carbohydrate antigens is challenging. Herein, we report a highly efficient and gree...

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Bibliographic Details
Published in:Green chemistry : an international journal and green chemistry resource : GC 2020-01, Vol.22 (22), p.8002-8011
Main Authors: Zhong, Kan, Ye, Jinfeng, Zhu, Xinhao, Cao, Hongzhi, Chang-Cheng, Liu
Format: Article
Language:English
Subjects:
Antigens
Blood groups
Carbohydrates
Cascade chemical reactions
Green chemistry
Intermediates
Monosaccharides
NMR
Nuclear magnetic resonance
Nucleotides
Oligosaccharides
Polysaccharides
Synthesis
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Summary:Sda antigens are a family of widely distributed histo-blood group antigens sharing the Siaα2,3(GalNAcβ1,4)Gal trisaccharide determinant. Despite their significant biological importance, the synthesis of these complex carbohydrate antigens is challenging. Herein, we report a highly efficient and green synthesis of diverse Sda antigens with different underlying structures and sialic acid forms utilizing 6 multienzyme cascade reaction systems. The multienzyme cascade approach combines sugar nucleotide donor generation enzymes and glycosyltransferases, providing an efficient and convenient approach for the rapid assembly of complex glycans from simple monosaccharide building blocks.
ISSN:1463-9262
1463-9270
DOI:10.1039/d0gc02971h