MTHFR genetic polymorphism and the risk of intrauterine fetal death in Polish women

• Published in: Ginekologia polska 2019-01, Vol.90 (2), p.76-81
• Main Authors: Wolski, Hubert, Kurzawinska, Grazyna, Drews, Krzysztof, Barlik, Magdalena, Kadziolka, Przemyslaw, Malewski, Zbyszko, Mikolajska-Ptas, Paula, Bylewski, Michal, Seremak-Mrozikiewicz, Agnieszka
• Format: Article
• Language: English
• Subjects: Age; Chronic illnesses; Eclampsia; Enzymes; Genotype & phenotype; Haplotypes; Investigations; Maternal & child health; Miscarriage; Molecular biology; Placental abruption; Polymerase chain reaction; Polymorphism; Preeclampsia; Pregnancy; Vitamin B; Womens health
• ISSN: 0017-0011; 2543-6767
• DOI: 10.5603/GP.2019.0013

OBJECTIVES: To evaluate the role of MTHFR genetic variants in the etiology of intrauterine fetal death in the second part of pregnancy at women from Polish population. MATERIAL AND METHODS: A case-control study was performed on a 76 women with a positive history of at least one in- trauterine fetal death after 22 gestational week and 400 healthy controls. The MTHFR genotyping for polymorphic sites 667C > T, 1298A > C, 1793G > A was determined by polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) method. RESULTS: For 1298A > C polymorphism, no statistically significant higher frequency of AA vs. AC+CC genotype was observed in the IUFD group 67.1 % vs. 55.2% in the control group (OR = 0.61, p = 0.05, pcorr = 0.15). We observed overrepresentation of three-locus haplotype CCG (p = 0.20; pcorr = 0.56) and two-locus haplotype CC (p = 0.17; pcorr = 0.48) in the IUFD group compared to controls. CONCLUSIONS: There was no observed relationships in genotype frequency of MTHFR 677C > T and 1793G > A variants, however 1298A > C showed a slightly higher but statistically insignificant prevalence in IUFD compared to the controls in Polish population. Further studies on a larger population are needed.