Survival of Methicillin-Resistant Staphylococcus aureus in Fish and Shrimp under Different Storage Conditions

• Published in: Journal of food protection 2020-05, Vol.83 (5), p.844-848
• Main Authors: Saklani, Pooja, Lekshmi, Manjusha, Nayak, Binaya Bhusan, Kumar, Sanath
• Format: Article
• Language: English
• Subjects: Animals; Antibiotics; Colony Count, Microbial; Drug resistance; Fish; Fishing; Food contamination; Food Handling; Food Microbiology; Food poisoning; Food safety; Food Storage - methods; Harpadon nehereus; Humans; Ice; Inoculation; Inoculum; Methicillin; Methicillin-Resistant Staphylococcus aureus - growth & development; Pathogens; Penicillin; Refrigeration; Seafood; Seafood - microbiology; Staphylococcus aureus; Staphylococcus infections; Storage conditions; Storage temperature; Survivability; Survival; Temperature
• ISSN: 0362-028X; 1944-9097
• DOI: 10.4315/JFP-19-546

Foods that are extensively handled during preparation and stored without refrigeration are often associated with staphylococcal food poisoning. This problem is more confounding when contaminating strains belong to the methicillin-resistant Staphylococcus aureus (MRSA) group. In this study, we investigated the survivability of MRSA in two seafood matrices under different storage conditions. MRSA was inoculated at 6 and 3 log CFU/g into all sample groups of peeled shrimp (Parapeneopsis stylifera) stored at -20°C, Bombay duck fish (Harpadon nehereus) stored in ice, and dried Bombay duck fish stored at 30 ± 2°C. The populations of MRSA in frozen peeled shrimp inoculated with MRSA at 6 log CFU/g were reduced by 1.52 log CFU/g, whereas in samples inoculated with 3 log CFU/g levels remained stable after 60 days of storage. In fresh Bombay duck fish inoculated with 6 log CFU/g and stored in ice for 18 days, MRSA levels decreased by 2.75 log CFU/g. In contrast, in fresh fish inoculated with 3 log CFU/g the total viable count increased by 3.02 log CFU/g over 16 days of ice storage. In dried fish stored at 30 ± 2°C, MRSA levels declined by 3.27 log CFU/g in samples inoculated with 6 log CFU/g and by 0.91 log CFU/g in samples inoculated with 3 log CFU/g. These results suggest that the survival of MRSA depends on the temperature of storage and the inoculum level. In our study, MRSA survival was higher when inoculated at 3 log CFU/g regardless of the seafood matrix and storage temperature.