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Down expression of LRP1B promotes cell migration via R ho A / C dc42 pathway and actin cytoskeleton remodeling in renal cell cancer
The low‐density lipoprotein receptor‐related protein 1B ( LRP 1B) is known as a putative tumor suppressor. The decreased expression of LRP 1B has been involved in multiple primary cancers in several studies. However, its expression and function in the carcinogenesis of renal cell cancer ( RCC ) rema...
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Published in: | Cancer science 2013-07, Vol.104 (7), p.817-825 |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The low‐density lipoprotein receptor‐related protein 1B (
LRP
1B) is known as a putative tumor suppressor. The decreased expression of
LRP
1B
has been involved in multiple primary cancers in several studies. However, its expression and function in the carcinogenesis of renal cell cancer (
RCC
) remain unclear. In this study, we investigated the expression of
LRP
1B
in
RCC
by
in situ
hybridization (
ISH
) and real‐time polymerase chain reaction (
qRT‐PCR
). Our results indicated that
LRP
1B
was frequently downexpressed in human
RCC
tissue and cell lines, which involved both epigenetic events (
DNA
methylation and histone deacetylation) and N‐terminal deletion of
LRP
1B
. Moreover, we testified that knockdown of
LRP
1B by sh
RNA
significantly promoted anchorage‐independent growth, cell migration and invasion in
HEK
293 cells and renal cancer cells 127
in vitro
. We further found that silencing of
LRP
1B
altered the expression of focal adhesion complex‐associated proteins, and Cdc42/RhoA activities, which regulate the cytoskeleton dynamics. Taken together, these results strongly support that
LRP
1B may function as a tumor suppressor against renal cell cancer, and may regulate cell motility via RhoA/Cdc42 pathway and actin cytoskeleton reorganization in
RCC
. |
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ISSN: | 1347-9032 1349-7006 |
DOI: | 10.1111/cas.12157 |