Next-generation sequencing in clinical virology: A review of its applications

Next-generation sequencing (NGS) has emerged as the most useful technique for wide-scale detection of infection-causing pathogens. Owing to the increasing demand for cost-effective and quick DNA sequencing protocols, many research groups have reported the use of NGS for lab research and experimentat...

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Bibliographic Details
Published in:Pure and applied biology 2020-12, Vol.9 (4), p.2356-2360
Main Author: Khan, Samea
Format: Article
Language:English
Subjects:
Coronaviruses
COVID-19
Culture media
Deoxyribonucleic acid
DNA
DNA sequencing
Drug resistance
Experimentation
Feasibility studies
Next-generation sequencing
Nucleotide sequence
Pathogens
Reviews
Virology
Viruses
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Summary:Next-generation sequencing (NGS) has emerged as the most useful technique for wide-scale detection of infection-causing pathogens. Owing to the increasing demand for cost-effective and quick DNA sequencing protocols, many research groups have reported the use of NGS for lab research and experimentation. NGS has revolutionized the field of DNA sequencing by helping sequence big data and overcomes the limitations associated with Sangers sequencing. In the current manuscript, we have aimed to review the feasibility of using NGS in public and clinical health laboratories for disease diagnostics. We believe that this review will add to the available literature by highlighting the important aspects of NGS in the characterization of various pathogens which cannot be grown using culture media and other conventional approaches.
ISSN:2304-2478
2304-2478
DOI:10.19045/bspab.2020.90250