Picea wilsonii NAC Transcription Factor PwNAC30 Negatively Regulates Abiotic Stress Tolerance in Transgenic Arabidopsis

NAC (NAM, ATAF1/2, and CUC2) transcription factors play important roles in the process of abiotic stress response in plants. However, little is known about the functional roles of NACs in Picea ( P. ) wilsonii . In this study, we functionally characterized a novel P. wilsonii NAC transcription facto...

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Bibliographic Details
Published in:Plant molecular biology reporter 2020-12, Vol.38 (4), p.554-571
Main Authors: Liang, Ke-hao, Wang, Ai-bin, Yuan, Yi-hang, Miao, Ya-hui, Zhang, Ling-yun
Format: Article
Language:English
Subjects:
Abiotic stress
Arabidopsis
Bioinformatics
Biomedical and Life Sciences
Drought
Gene expression
Life Sciences
Localization
Metabolomics
Original Paper
Plant Breeding/Biotechnology
Plant Sciences
Plant tissues
Proteomics
Reactive oxygen species
Salinity tolerance
Seedlings
Stamens
Transcription factors
Transgenic plants
Woody plants
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Summary:NAC (NAM, ATAF1/2, and CUC2) transcription factors play important roles in the process of abiotic stress response in plants. However, little is known about the functional roles of NACs in Picea ( P. ) wilsonii . In this study, we functionally characterized a novel P. wilsonii NAC transcription factor PwNAC30 by heterologous expression in Arabidopsis . The results showed that PwNAC30 is mainly localized in the nucleus by transient expression in Nicotiana benthamiana and can function as a nuclear localization transcription factor. β-Glucuronidase (GUS) staining in transgenic Arabidopsis ( PwNAC30-promoter-GUS ) confirmed the expression pattern of PwNAC30 throughout the plant development process, which is expressed in most of the plant tissues except stamens and petals. Overexpression of PwNAC30 in Arabidopsis significantly repressed the tolerance of seedlings and mature plants to both drought and salt stresses, while exerting no influence on growth and development of plants. The accumulation of reactive oxygen species (ROS) was significantly increased in transgenic plants, and the expression of some stress-responsive genes was obviously inhibited in PwNAC30 overexpression lines. Our study reveals that PwNAC30 functions as a negative regulator of plant tolerance to drought or salt stress, which provides new insights into abiotic tolerance mechanisms in woody plants.
ISSN:0735-9640
1572-9818
DOI:10.1007/s11105-020-01216-z