Naturally enriched 13C breath test as a carbohydrate intake marker in obese women

• Published in: Nutrire : revista de Sociedade Brasileira de Alimentação e Nutrição = journal of the Brazilian Society of Food and Nutrition 2020-07, Vol.45 (2), Article 17
• Main Authors: Beato, Gabriel Cunha, Ravelli, Michele Novaes, Sartori, Maria Márcia Pereira, de Oliveira, Maria Rita Marques
• Format: Article
• Language: English
• Subjects: Biomarkers; Breath tests; Carbohydrates; Clinical Nutrition; Food Science; Glucose; Medicine; Medicine & Public Health; Metabolism; Nutrition; Obesity; Womens health
• ISSN: 2316-7874; 1519-8928; 2316-7874
• DOI: 10.1186/s41110-020-00119-z

Background The 13 C breath test has been proposed as a potential biomarker for the evaluation of metabolism and carbohydrate intake. To study the dynamics of the use of substrates in human metabolism is especially important for glucose metabolism in obese people. Purpose This study aimed to verify the use of 13 C in a naturally enriched corn glucose liquid meal through the breath test as a potential marker of energy metabolism in women with a body mass index (BMI) above 35 kg /m 2 . Methods Twenty-five woman (13 non-obese; 12 obese) participated in the study. The breath samples were collected in tubes prior to consumption of the standard liquid meal and at 30-min intervals for 6 h and analyzed in a continuous flow isotope ratio mass spectrometer. The curves of breath isotopic enrichment were adjusted by the Boltzmann sigmoidal regression model, and the t test was used to evaluate differences between the means of the curves. Results It was found a faster metabolic turn-over of the 13 C-glucose in obese women. Conclusion The breath test using naturally enriched stable isotopes has potential to be used as an energy metabolism marker in subjects with different BMI.