Biosynthesis of Silver Nanoparticles by Methylophilus quaylei, Characterization and Its Impact on Established Biofilms

In this study, Methylophilus quaylei proved to possess the ability to reduce AgNO 3 and produce silver nanoparticles (AgNPs) under specific conditions via bacterial cells and cell-free supernatant (CFS). The obtained AgNPs via bacterial cells were examined by the transmission of electron microscopy...

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Bibliographic Details
Published in:BioNanoScience 2020-12, Vol.10 (4), p.885-898
Main Authors: Mohamed, Abir M. H. A., Sorokin, Vladimir V., Skladnev, Dmitry A., Shevlyagina, Natalia V., Zhukhovitsky, Vladimir G., Pshenichnikova, Anna B.
Format: Article
Language:English
Subjects:
Abnormalities
Absorption spectroscopy
Biofilms
Biological and Medical Physics
Biomaterials
Biophysics
Biosynthesis
Circuits and Systems
Engineering
Light scattering
Methylophilus
Minimum inhibitory concentration
Nanoparticles
Nanotechnology
Photon correlation spectroscopy
Polypropylene
Scanning electron microscopy
Silver
Silver nitrate
Strains (organisms)
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Summary:In this study, Methylophilus quaylei proved to possess the ability to reduce AgNO 3 and produce silver nanoparticles (AgNPs) under specific conditions via bacterial cells and cell-free supernatant (CFS). The obtained AgNPs via bacterial cells were examined by the transmission of electron microscopy (TEM) consistent with X-ray microanalysis and for AgNPs via CFS by visible (UV–Vis) absorption spectroscopy, scanning electron microscopy (SEM), and dynamic light scattering (DLS). The purpose of this study was to evaluate the antibiofilm effect of AgNPs. The effectiveness of the minimum inhibitory concentration (MIC) of AgNPs in supplementation with CFS was determined on established biofilms on polypropylene by the number of colony forming unit (CFU). These nanoparticles exhibit an antibiofilm effect. CFS was tested individually and in supplementation with the MIC AgNPs obtained on established biofilms of M. quaylei MT and M. quaylei SM. The CFS individually decreased established biofilm for both strains. Further, the obtained MIC of AgNPs in combination with CFS exhibited significant antibiofilm effect against established biofilms for both strains by the destruction of biofilm architecture and detecting cell morphological abnormalities that were observed by SEM. These results showed that the obtained AgNPs had a strong antibiofilm effect against the examined strains.
ISSN:2191-1630
2191-1649
DOI:10.1007/s12668-020-00780-8