Autoimmune Markers for Non-Invasive Diagnosis of Endometriosis in Women

• Published in: Biochemistry (Moscow). Supplement. Series B, Biomedical chemistry Biomedical chemistry, 2020-10, Vol.14 (4), p.335-339
• Main Authors: Menzhinskaya, I. V., Melkumyan, A. G., Pavlovich, S. V., Chuprynin, V. D., Vanko, L. V., Sukhikh, G. T.
• Format: Article
• Language: English
• Subjects: 17β-Estradiol; Antibodies; Antigens; Autoantibodies; Bioorganic Chemistry; Chemistry; Chemistry and Materials Science; Chorionic gonadotropin; Chronic illnesses; Diagnosis; Endometriosis; Endometrium; Enzyme-linked immunosorbent assay; Enzymes; Estrogens; Gonadotropins; Immune response; Immunoglobulin G; Inflammation; Medicinal Chemistry; Phosphopyruvate hydratase; Pituitary (anterior); Progesterone; Steroid hormones; Tropomyosin
• ISSN: 1990-7508; 1990-7516
• DOI: 10.1134/S1990750820040083

Endometriosis is a common estrogen-dependent chronic disease of women of reproductive age; it is associated with dysregulation of the immune response, local inflammatory process and increased autoantibody production. The purpose of this study was to investigate the autoantibody profile in women with endometriosis by means of new modifications of the enzyme-linked immunosorbent assay and to evaluate their diagnostic value. In women with external genital endometriosis stage III–IV ( n = 39), a wide range of autoantibodies of mainly class G was detected; these included antibodies to endometrial antigens (tropomyosin 3, tropomodulin 3), the enzyme α-enolase, steroid hormones (estradiol, progesterone) and gonadotropin. Moreover, the detection rate of IgG antibodies to tropomyosin 3, α-enolase, estradiol, and human chorionic gonadotropin and their levels in patients with endometriosis were higher than in healthy women ( n = 26) ( p  < 0.05). IgG antibodies to tropomyosin 3, α-enolase and estradiol were characterized by a higher diagnostic value for endometriosis. When determining the combination of these antibodies, the diagnostic significance increased, the AUC value was 0.875 [0.772–0.978] ( p < 0.0001), and sensitivity and specificity reached 83.3%. Thus, autoantibodies to tropomyosin 3, α-enolase and estradiol are promising for inclusion in the panel of biomarkers for non-invasive diagnosis of endometriosis.