In vitro culture method of Ditylenchus destructor (Tylenchida: Anguinidae) using garlic

Ditylenchus destructor Thorne (Tylenchida: Anguinidae) is a plant-parasitic nematode that causes garlic rot in Japan. A method that can be used to culture the nematode on garlic has not yet been established, but is required to be able to conduct studies for a better understanding of the nematode’s p...

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Bibliographic Details
Published in:Applied entomology and zoology 2020-11, Vol.55 (4), p.435-437
Main Author: Yoshiga, Toyoshi
Format: Article
Language:English
Subjects:
Agriculture
Anguinidae
Applied Ecology
Biomedical and Life Sciences
Ditylenchus destructor
Entomology
Environmental Management
Garlic
Inoculation
Life Sciences
Nematodes
Plant Pathology
Rot
Technical Note
Tylenchida
Zoology
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Summary:Ditylenchus destructor Thorne (Tylenchida: Anguinidae) is a plant-parasitic nematode that causes garlic rot in Japan. A method that can be used to culture the nematode on garlic has not yet been established, but is required to be able to conduct studies for a better understanding of the nematode’s physiology and ecology. The purpose of this study was to develop such a culture method. Surface-sterilized nematodes (200 individuals) were inoculated onto a surface-sterilized garlic storage leaf on 1% agar in a 100 ml medium bottle, and incubated at 25 °C. Seven weeks after inoculation, a mean number of approximately 360,000 axenic nematodes per bottle was obtained. The culture method established in this study is simple, less laborious, and can be useful for the studies of D. destructor associated with garlic.
ISSN:0003-6862
1347-605X
DOI:10.1007/s13355-020-00701-x