Gamma Radiation Tolerance and Protein Carbonylation Caused by Irradiation of Resting Cysts in the Free-living Ciliated Protist Colpoda cucullus

• Published in: Acta protozoologica 2020-01, Vol.59 (2), p.67-75
• Main Authors: Saito, Ryota, Koizumi, Ryota, Sakai, Tatsuya, Shimizu, Taiga, Ono, Taiki, Sogame, Yoichiro
• Format: Article
• Language: English
• Subjects: Colpoda; Colpoda cucullus; Cysts; Desiccation; Environmental stress; Excystment; Gamma radiation; Gamma rays; Incubation period; Irradiation; Morphology; Proteins; Reactive oxygen species; Survival; Terrestrial environments; Vegetative cells; γ Radiation
• ISSN: 0065-1583; 1689-0027
• DOI: 10.4467/16890027AP.20.006.12674

The ciliate Colpoda cucullus forms resting cysts to survive unfavorable environmental stresses. In this study, we have shown that Colpoda resting cysts survived exposure to a gamma radiation dose of 4000 Gy, although vegetative cells were killed by 500 Gy. After 4000 Gy irradiation, more than 90% of resting cysts and approximately 70% of dry cysts could excyst to form vegetative cells. In both cases, the excystment gradually increased after the induction of excystment. In addition, we also showed that protein carbonylation level was increased by gamma irradiation, but decreased by incubation in the cyst state. These results indicated that cell damage was repaired in resting cysts. Colpoda probably developed tolerance to gamma radiation by forming resting cysts as a strategy for growth in terrestrial environments, as part of contending with the stress due to reactive oxygen species caused by desiccation.