Protective effect of 2-dodecyl-6-methoxycyclohexa-2, 5-diene-1, 4-dione, isolated from Averrhoa carambola L., against Aβ1–42-induced apoptosis in SH-SY5Y cells by reversing Bcl-2/Bax ratio

• Published in: Psychopharmacology 2021, Vol.238 (1), p.193-200
• Main Authors: Lu, Shunyu, Wei, Xiaojie, Zhang, Hongliang, Chen, Zhenfeng, Li, Juman, Xu, Xiaohui, Xie, Qiuqiao, Chen, Lixiu, Ye, Fangxing, Phama, Hoa Thi Thai, Jiang, Luhui, Huang, Tianmin, Wei, Jinbin, Huang, Renbin
• Format: Article
• Language: English
• Subjects: Alzheimer Disease - pathology; Alzheimer's disease; Amyloid beta-Peptides - toxicity; Apoptosis; Apoptosis - drug effects; Averrhoa - chemistry; BAX protein; Bcl-2 protein; bcl-2-Associated X Protein - metabolism; Biomedical and Life Sciences; Biomedicine; Caspase 3 - metabolism; Caspase-3; Cell Line, Tumor; Cell Survival - drug effects; Cell viability; Cholecystokinin; Cyclohexenes - isolation & purification; Cyclohexenes - pharmacology; Cytochrome; Cytochrome c; Drugs, Chinese Herbal - isolation & purification; Drugs, Chinese Herbal - pharmacology; Flow cytometry; Humans; Membrane potential; Membrane Potential, Mitochondrial - drug effects; Mitochondria; Neurodegenerative diseases; Neurosciences; Neurotoxicity; Original Investigation; Peptide Fragments - toxicity; Pharmacology/Toxicology; Proto-Oncogene Proteins c-bcl-2 - metabolism; Psychiatry; Transmission electron microscopy
• ISSN: 0033-3158; 1432-2072
• DOI: 10.1007/s00213-020-05668-9

Background and purpose Aβ1–42-induced neurotoxicity has been considered as a possible mechanism to aggravate the onset and progression of Alzheimer’s disease (AD). In this study, we aim to determine the protective effect of DMDD on the apoptosis of SH-SY5Y cells induced by Aβ1–42 and elucidate potential mechanism of DMDD’s protective function in apoptosis. Experimental approach CCK-8, AnnexinV-FITC/PI flow cytometry, and transmission electron microscopy analysis were used to determine the protection of DMDD on Aβ1–42-evoked apoptosis of SH-SY5Y cells. Cytochrome c release, JC-1 staining, and measuring the protein of Bcl-2 family by Western blot were applied to elucidate the mechanism of DMDD’s protective function in apoptosis. Key results Three concentration of DMDD (5 μmol/L, 10 μmol/L, and 20 μmol/L) rescues the cell viability loss and apoptosis of SH-SY5Y cells cultivated in Aβ1–42. The expressions of cleaved Caspase-3, -8, -9, the cytochrome c release, and mitochondrial membrane potential loss were inhibited by DMDD in Aβ1–42-insulted SH-SY5Y cells. The Western blot analysis showed that DMDD pretreatment clearly downregulated the protein of Bax and upregulated Bcl-2. Moreover, the Bcl-2/Bax ratio was obviously decreased in cells only exposed to Aβ1–42, but, which was suppressed by treated with DMDD. Conclusion and implications DMDD attenuated the apoptosis of SH-SY5Y cells induced by Aβ1–42 through reversing the Bcl-2/Bax ratio.