Membrane transporters: the key drivers of transport of secondary metabolites in plants

• Published in: Plant cell reports 2021, Vol.40 (1), p.1-18
• Main Authors: Gani, Umar, Vishwakarma, Ram A., Misra, Prashant
• Format: Article
• Language: English
• Subjects: Alkaloids - metabolism; ATP-Binding Cassette Transporters - metabolism; Biocompatibility; Biological Transport; Biomedical and Life Sciences; Biotechnology; Cell Biology; Cytotoxicity; Economic importance; Extrusion; Herbivory; Life Sciences; Medicinal plants; Membrane Transport Proteins - genetics; Membrane Transport Proteins - metabolism; Membranes; Metabolic engineering; Metabolic Engineering - methods; Metabolism; Metabolites; Molecular modelling; Organs; Plant Biochemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Sciences; Plants - genetics; Plants - metabolism; Plants, Genetically Modified; Protein transport; Proteins; Review; Secondary Metabolism; Secondary metabolites; Terpenes - metabolism; Toxicity
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-020-02599-9

Key message This review summarizes the recent updates in the area of transporters of plant secondary metabolites, including their applied aspects in metabolic engineering of economically important secondary metabolites. Plants have evolved biosynthetic pathways to produce structurally diverse secondary metabolites, which serve distinct functions, including defense against pathogens and herbivory, thereby playing a pivotal role in plant ecological interactions. These compounds often display interesting bioactivities and, therefore, have been used as repositories of natural drugs and phytoceuticals for humans. At an elevated level, plant secondary metabolites could be cytotoxic to the plant cell itself; therefore, plants have developed sophisticated mechanisms to sequester these compounds to prevent cytotoxicity. Many of these valuable natural compounds and their precursors are biosynthesized and accumulated at diverse subcellular locations, and few are even transported to sink organs via long-distance transport, implying the involvement of compartmentalization via intra- and intercellular transport mechanisms. The transporter proteins belonging to different families of transporters, especially ATP binding cassette (ABC) and multidrug and toxic compound extrusion (MATE) have been implicated in membrane-mediated transport of certain plant secondary metabolites. Despite increasing reports on the characterization of transporter proteins and their genes, our knowledge about the transporters of several medicinally and economically important plant secondary metabolites is still enigmatic. A comprehensive understanding of the molecular mechanisms underlying the whole route of secondary metabolite transportome, in addition to the biosynthetic pathways, will aid in systematic and targeted metabolic engineering of high-value secondary metabolites. The present review embodies a comprehensive update on the progress made in the elucidation of transporters of secondary metabolites in view of basic and applied aspects of their transport mechanism.