A green analytical method for the analysis of polycyclic aromatic hydrocarbons in oral fluids from crack smokers

Develop and validate a method of solid-phase microextraction (SPME) and liquid chromatography to investigate three major polycyclic aromatic hydrocarbons (PAHs) in oral fluid. The extraction phase was exposed to 1.5 ml of diluted oral fluid under stirring at 1000 rpm for 60 min, at 70°C. Then, it wa...

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Bibliographic Details
Published in:Bioanalysis 2020-12, Vol.12 (24), p.1711-1724
Main Authors: Rossiter, Pavla Lôbo, de Alencar Danda, Lucas José, Pimentel, Maria Fernanda, Almeida Silva de Santana, Danielle Cristine, Malagueño de Santana, Fernando José
Format: Article
Language:English
Subjects:
Acetonitrile
Chromatography
Cocaine
crack cocaine
Drugs
Investigations
Liquid chromatography
Mutagenesis
oral fluid
Oral fluids
polycyclic aromatic hydrocarbon
Polycyclic aromatic hydrocarbons
Smoking
Solid phase methods
solid-phase microextraction
Tobacco
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Summary:Develop and validate a method of solid-phase microextraction (SPME) and liquid chromatography to investigate three major polycyclic aromatic hydrocarbons (PAHs) in oral fluid. The extraction phase was exposed to 1.5 ml of diluted oral fluid under stirring at 1000 rpm for 60 min, at 70°C. Then, it was immersed in 200 μl of acetonitrile for 10 min at 25°C for desorption of the analytes. Linearity, absolute recovery, and inter- and intra-assay relative standard deviations and relative errors were 50–300 ng.ml , ≥24% and ≤15% for all analytes, respectively. A full factorial design was used to SPME optimization. The method is suitable for the exploratory analysis of some PAHs in the oral fluid of crack smokers.
ISSN:1757-6180
1757-6199
DOI:10.4155/bio-2020-0251