qPCR assay for the detection of pseudocowpox virus

Pseudocowpox is a zoonosis caused by pseudocowpox virus (PCPV), which mainly affects cows but can be an occupational disease of humans. The aim of the study was to validate a quantitative polymerase chain reaction (qPCR) assay for the detection of PCPV. The assay was able to detect up to 1000 copies...

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Bibliographic Details
Published in:Archives of virology 2021, Vol.166 (1), p.243-247
Main Authors: de Oliveira Lopes, Gabriel Augusto, Ferreira, Luciana Rabello, de Souza Trindade, Giliane, Fonseca, Antônio Augusto, dos Reis, Jenner Karlisson Pimenta
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Brief Report
Cattle
Cattle Diseases - virology
Cattle industry
Cross-reactivity
Economic impact
Foot & mouth disease
Genomes
Humans
Infectious Diseases
Laboratories
Medical Microbiology
Occupational diseases
Polymerase chain reaction
Polymerase Chain Reaction - methods
Poxviridae Infections - veterinary
Poxviridae Infections - virology
Pseudocowpox Virus - genetics
Reproducibility
Reproducibility of Results
Sensitivity and Specificity
Virology
Viruses
Zoonoses
Zoonoses - virology
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Summary:Pseudocowpox is a zoonosis caused by pseudocowpox virus (PCPV), which mainly affects cows but can be an occupational disease of humans. The aim of the study was to validate a quantitative polymerase chain reaction (qPCR) assay for the detection of PCPV. The assay was able to detect up to 1000 copies of PCPV per µL in field samples, with a sensitivity of 80% and a specificity of 100%. We did not observe any cross-reactivity between PCPV-positive samples and samples that were positive for other genetically similar viruses. The repeatability and reproducibility were adequate according to parameters preestablished in official test validation manuals.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-020-04872-4