Cytotoxic effect of aminoglycoside antibiotics on the mammalian cell lines

Aminoglycoside antibiotics have been used for treating serious but also routine infections in veterinary and human medicine for many years. The basic aim of this work is to evaluate the cytotoxicity of dihydrostreptomycin and neomycin in vitro on three cell cultures - BHK-21 (Syrian golden hamster k...

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Published in:Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering Toxic/hazardous substances & environmental engineering, 2021, Vol.56 (1), p.1-8
Main Authors: Kovacik, Anton, Tvrda, Eva, Jambor, Tomas, Fulopova, Diana, Kovacikova, Eva, Hleba, Lukas, Kołodziejczyk, Łukasz M., Hlebova, Miroslava, Gren, Agnieszka, Massanyi, Peter
Format: Article
Language:English
Subjects:
Aminoglycoside antibiotics
Aminoglycosides
Animals
Anti-Bacterial Agents - administration & dosage
Anti-Bacterial Agents - toxicity
Antibiotics
BHK21
Biotechnology
Cats
Cell Line
Cell lines
Cell Survival - drug effects
Cell viability
Chlorocebus aethiops
Cricetinae
Cytotoxicity
Dihydrostreptomycin
Dihydrostreptomycin Sulfate - administration & dosage
Dihydrostreptomycin Sulfate - toxicity
Dose-Response Relationship, Drug
Embryo fibroblasts
Evaluation
FEA
Fibroblasts
Fibroblasts - drug effects
Fibroblasts - pathology
Finite element method
Fluorescence
Humans
Kidneys
MTT assay
Neomycin
Neomycin - administration & dosage
Neomycin - toxicity
Toxicity
VERO
Vero Cells
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Summary:Aminoglycoside antibiotics have been used for treating serious but also routine infections in veterinary and human medicine for many years. The basic aim of this work is to evaluate the cytotoxicity of dihydrostreptomycin and neomycin in vitro on three cell cultures - BHK-21 (Syrian golden hamster kidney fibroblast), VERO (African green monkey kidney fibroblast) and FEA (feline embryonic fibroblast) cells. The morphological changes were examined by Giemsa staining. Cells were dried and visualized under fluorescence microscope. After the exposure to different experimental doses of dihydrostreptomycin (812.5-20000 µg/mL) and neomycin (1000-20000 µg/mL) during 24 h, the viability of BHK-21, FEA and VERO cell lines were evaluated by MTT assay. Viability of BHK-21 cells significantly (P 
ISSN:1093-4529
1532-4117
DOI:10.1080/10934529.2020.1830653