Culturing of Melanocytes from the Equine Hair Follicle Outer Root Sheath

Hair follicles harbor a heterogeneous regenerative cell pool and represent a putative low-to-non-invasively available source of stem cells. We previously reported a technology for culturing human melanocytes from the hair follicle outer root sheath (ORS) for autologous pigmentation of tissue enginee...

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Bibliographic Details
Published in:Processes 2021-01, Vol.9 (1), p.177
Main Authors: Li, Hanluo, Michler, Jule Kristin, Bartella, Alexander, Sander, Anna Katharina, Gaus, Sebastian, Hahnel, Sebastian, Zimmerer, Rüdiger, Simon, Jan-Christoph, Savkovic, Vuk, Lethaus, Bernd
Format: Article
Language:English
Subjects:
Autografts
Cell culture
Comparative studies
Cultivation
Cytoskeleton
Fibroblasts
Follicles
Hair
Horses
Hypoxia
In vitro methods and tests
Melanin
Melanocytes
Melanosomes
Morphology
Pigmentation
Sheaths
Stem cells
Tyrosinase
Vimentin
Vitiligo
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Summary:Hair follicles harbor a heterogeneous regenerative cell pool and represent a putative low-to-non-invasively available source of stem cells. We previously reported a technology for culturing human melanocytes from the hair follicle outer root sheath (ORS) for autologous pigmentation of tissue engineered skin equivalents. This study translated the ORS technology to horses. We de-veloped a culture of equine melanocytes from the ORS (eMORS) from equine forelock hair follicles cultured by means of an analogue human hair follicle-based in vitro methodology. The procedure was adjusted to equine physiology by addition of equine serum to the culture medium. The hair follicles were isolated by macerating forelock skin rests, enzymatically digested and subjected to air-medium-interface cultivation method. The procedure resulted in differentiated equine melanocytes, which exhibited typical morphology, presence of melanosomes, expression of cytoskeleton proteins vimentin, α-SMA, Sox2, S100ß and tyrosinase as well as tyrosinase activity followed by production of melanin. According to all assessed parameters, eMORS could be ranked as partially melanotic melanocytes. The results of the study offer an experimental base for further insight into hair follicle biology in equine and for comparative studies of hair follicles across different species.
ISSN:2227-9717
2227-9717
DOI:10.3390/pr9010177