High-Performance Liquid Chromatography Determination of Free Sugars and Mannitol in Mushrooms Using Corona Charged Aerosol Detection

Refractive index detector is usually used in the analysis of sugars in mushrooms, which is characterized by poor sensitivity, reproducibility, and susceptibility to interference from co-eluting sample components. In the current study, identification and determination of free sugars in mushroom sampl...

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Bibliographic Details
Published in:Food analytical methods 2021-02, Vol.14 (2), p.209-216
Main Authors: Sławińska, Aneta, Jabłońska-Ryś, Ewa, Stachniuk, Anna
Format: Article
Language:English
Subjects:
Acetonitrile
Aerosols
Analytical Chemistry
Basidiocarps
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Chromatography
Flow rates
Flow velocity
Food Science
High performance liquid chromatography
Linearity
Liquid chromatography
Mannitol
Microbiology
Mushrooms
Refractivity
Reproducibility
Sugar
Trehalose
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Summary:Refractive index detector is usually used in the analysis of sugars in mushrooms, which is characterized by poor sensitivity, reproducibility, and susceptibility to interference from co-eluting sample components. In the current study, identification and determination of free sugars in mushroom samples by high-performance liquid chromatography coupled to corona charged aerosol detector (HPLC-CAD) were presented for the first time. The best chromatographic separation was performed on a Shodex Asahipak NH2P-50 4E 5 μm and mobile phase composed of 75% acetonitrile and 25% water with flow rate was 1 mL/min. The developed method offers good linearity in concentration range 0.001–0.01 or 0.01–0.2 mg/mL for tested compounds with R 2  > 0.99. Limit of detection (LOD) for analytes was in the range of 7.1–120.2 ng on column. HPLC-CAD method showed very good reproducibility (RSD 
ISSN:1936-9751
1936-976X
DOI:10.1007/s12161-020-01863-8