Novel Imaging Modalities Shedding Light on Plant Biology: Start Small and Grow Big

• Published in: Annual review of plant biology 2020-04, Vol.71 (1), p.789-816
• Main Authors: Clark, Natalie M, Van den Broeck, Lisa, Guichard, Marjorie, Stager, Adam, Tanner, Herbert G, Blilou, Ikram, Grossmann, Guido, Iyer-Pascuzzi, Anjali S, Maizel, Alexis, Sparks, Erin E, Sozzani, Rosangela
• Format: Article
• Language: English
• Subjects: Biology; Computer applications; Datasets; Förster resonance energy transfer; Imaging; imaging of macroscopic traits; light sheet microscopy; microfluid devices; multiscale imaging techniques; Parallel processing; Plant Development; Plant growth; Plants; scanning fluorescent correlation spectroscopy; Spatial discrimination; Spatial resolution
• ISSN: 1543-5008; 1545-2123
• DOI: 10.1146/annurev-arplant-050718-100038

The acquisition of quantitative information on plant development across a range of temporal and spatial scales is essential to understand the mechanisms of plant growth. Recent years have shown the emergence of imaging methodologies that enable the capture and analysis of plant growth, from the dynamics of molecules within cells to the measurement of morphometricand physiological traits in field-grown plants. In some instances, these imaging methods can be parallelized across multiple samples to increase throughput. When high throughput is combined with high temporal and spatial resolution, the resulting image-derived data sets could be combined with molecular large-scale data sets to enable unprecedented systems-level computational modeling. Such image-driven functional genomics studies may be expected to appear at an accelerating rate in the near future given the early success of the foundational efforts reviewed here. We present new imaging modalities and review how they have enabled a better understanding of plant growth from the microscopic to the macroscopic scale.