Differences in the Manifestation of Cell Pluripotence In Vivo and In Vitro in the Mutant Arabidopsis thaliana with the Phenotype of Cell Memory Disorder

• Published in: Russian journal of plant physiology 2021, Vol.68 (1), p.46-55
• Main Authors: Kupriyanova, E. V., Denisova, E. R., Baier, M. A., Ezhova, T. A.
• Format: Article
• Language: English
• Subjects: Arabidopsis thaliana; Biomedical and Life Sciences; Cell culture; Cell proliferation; Epigenetics; Gene expression; Genes; Hormones; Leaves; Life Sciences; Meristems; Mutants; Phenotypes; Plant cells; Plant Physiology; Plant Sciences; Pluripotency; Regeneration; Research Papers; Shoots; Tissue culture
• ISSN: 1021-4437; 1608-3407
• DOI: 10.1134/S1021443721010106

Plant cells cultivated in vitro are a convenient model for studying the genetic and physiological mechanisms necessary for the cells to acquire a state of pluripotency. Earlier studies on a model plant Arabidopsis thaliana (L.) Heynh. have identified the key role of genes that determine the pluripotency of cells in the shoot apical meristem in de novo shoot regeneration in tissue culture. In accordance with this, cells of mutant plants with a higher level of expression of pluripotency genes were characterized by an increased potential for de novo shoot regeneration. The tae mutant was the exception to this rule. The mutant resumed the expression of pluripotency genes and cell proliferation at the late stages of leaf development, which indicates a violation of the mechanisms for maintaining epigenetic cellular memory. At the same time, leaf cells cultured in vitro showed a lower proliferative activity compared to the wild type and were not capable of de novo regeneration of shoots. A decrease in the regenerative potential of cultured cells of the tae mutant indicates an important role of epigenetic memory in the response of cells to exogenous hormones. Impaired epigenetic memory of leaf cells of the tae  mutant and differences in their proliferative and regenerative capacities in planta and in vitro make this mutant a unique model for studying the role of epigenetic modifications in the regulation of cell pluripotency.