Development of potentıometrıc bıosensor for dıagnosıs of prostate cancer

• Published in: Materials science & engineering. B, Solid-state materials for advanced technology Solid-state materials for advanced technology, 2021-01, Vol.263, p.114789, Article 114789
• Main Authors: Altunkök, Nazire, Biçen Ünlüer, Özlem, Birlik Özkütük, Ebru, Ersöz, Arzu
• Format: Article
• Language: English
• Subjects: ANADOLUCA method; Antibodies; Biosensor; Biosensors; GFOX; Prostate cancer; Public health; Response time; Sarcosine; Selectivity
• ISSN: 0921-5107; 1873-4944
• DOI: 10.1016/j.mseb.2020.114789

[Display omitted] •Nano anti-sarcosine antibody was synthesized for the determination of sarcosine. Prostate cancer is an important public health problem in the world with its burden of disease, increase in fatality and incidence rates. Recently, naturally occurring amino acid sarcosine has been investigated as a recognized marker of prostate cancer. The aim of the study for the early detection of prostate cancer is to develop a method of high sensitivity, low cost and low concentration in the determination of sarcosine. High sensitive nano anti-sarcosine-antibody and nano anti-sarcosine antibody-GFOX polymer have been prepared for the potentiometric detection of sarcosine and its reusability as biosensors have investigated. Nano anti-sarcosine antibody and nano anti-sarcosine antibody-GFOX potentiometric biosensor have shown excellent detection performance. Nano anti-sarcosine antibody potentiometric biosensor and nano anti-sarcosine antibody-GFOX potentiometric biosensor have low detection limit (4.75 × 10−9 mM and 3.34 × 10−9mM, respectively), high selectivity, short response time, wide linear range, and satisfactory long-term stability (3–4 months) for sarcosine detection.