Determination of Phenolic Acids in Botanical Pharmaceutical Products by Capillary Electrophoresis with Chemiluminescence Detection

A rapid and simple method was developed to determine the phenolic acids ferulic acid, chlorogenic acid and salicylic acid in botanical drugs by capillary electrophoresis with chemiluminescence detection. The sensitive chemiluminescence detection was carried out in a laboratory-constructed temperatur...

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Bibliographic Details
Published in:Analytical letters 2021-03, Vol.54 (5), p.817-829
Main Authors: Chen, Xin, Mao, Jingxiu, Wen, Fuyu, Xu, Xueqin
Format: Article
Language:English
Subjects:
Acids
botanical drugs
Capillary electrophoresis
Chemiluminescence
Chlorogenic acid
Electrophoresis
Ferulic acid
Phenolic acids
Potassium permanganate
Reagents
Salicylic acid
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Summary:A rapid and simple method was developed to determine the phenolic acids ferulic acid, chlorogenic acid and salicylic acid in botanical drugs by capillary electrophoresis with chemiluminescence detection. The sensitive chemiluminescence detection was carried out in a laboratory-constructed temperature-controlled interface by the use of acidic potassium permanganate as a chemiluminescence reagent. To obtain the optimized conditions, the experimental conditions for the capillary electrophoresis separation and chemiluminescence detection were systematically examined. Under the optimized conditions, the analytes were well separated within 7 min. The limits of detection for ferulic acid, chlorogenic acid and salicylic acid were 35, 58 and 37 µg/mL and the limits of quantification were 117, 193 and 123 µg/mL, respectively. The proposed method was successfully applied for the quantitative determination of phenolic acids in Lonicera japonica flos and Rhizoma Chuanxiong.
ISSN:0003-2719
1532-236X
DOI:10.1080/00032719.2020.1783675