Smartphone based highly sensitive visualized detection of acid phosphatase enzyme activity

With the rapid development of point-of-care (POC) technologies, development of sensitive method featured with fast analysis and affordable devices has become an emerging requirement for practical applications. In this study, we introduced a smartphone-based RGB analysis system for the sensitive dete...

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Bibliographic Details
Published in:Analytical methods 2021-02, Vol.13 (6), p.89-816
Main Authors: Li, Rui, Sun, Yanan, Jin, Lihua, Qiao, Xiaohong, Li, Cong, Shen, Yehua
Format: Article
Language:English
Subjects:
Acid Phosphatase
Acids
Alkaline Phosphatase
Ascorbic acid
Biological properties
Biological samples
Core-shell structure
Enzymatic activity
Enzyme activity
Enzymes
Gold
Morphology
Nanostructures
Phosphatase
Silver
Smartphone
Smartphones
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Summary:With the rapid development of point-of-care (POC) technologies, development of sensitive method featured with fast analysis and affordable devices has become an emerging requirement for practical applications. In this study, we introduced a smartphone-based RGB analysis system for the sensitive detection of acid phosphatase (ACP) enzyme activity. In the presence of ACP, l -ascorbic acid 2-phosphate (AAP) can be converted into ascorbic acid (AA), which can reduce Ag + to Ag 0 and format the Au@Ag core-shell nanostructure. This morphology change of the Au@Ag core-shell would trigger a significant color variation (pink to yellow). A good linear relationship between the RGB model parameter and the concentration of ACP could be obtained with a detection limit of 0.1 U L −1 . Moreover, this sensing strategy is suitable for the detection of ACP in practical serum samples. Thus, this simple but powerful protocol has great potential application for on-site detection of ACP in future complex biological samples. A smartphone-based RGB analysis system was constructed for the sensitive detection of acid phosphatase (ACP) enzyme activity.
ISSN:1759-9660
1759-9679
DOI:10.1039/d0ay02128h