Enzymatic pathway involved in the degradation of fluoranthene by microalgae Chlorella vulgaris

In the present study, the potential of a microalga, Chlorella vulgaris , was assessed for the bioremediation of fluoranthene (FLT), a four ring polycyclic aromatic hydrocarbon (PAH). With an initial cell density of C. vulgaris (OD 680  = 0.100), 54–58% of 25 μM FLT was removed from the growth medium...

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Bibliographic Details
Published in:Ecotoxicology (London) 2021-03, Vol.30 (2), p.268-276
Main Authors: Tomar, Rupal Singh, Jajoo, Anjana
Format: Article
Language:English
Subjects:
Algae
Analysis
Aromatic compounds
Aromatic hydrocarbons
Biodegradation
Biomass
Bioremediation
Carbohydrates
Carotenoids
Catechin
Catechol
Cell culture
Cell density
Chlorella
Chlorella vulgaris
Chlorophyll
Chlorophylls
Dioxygenase
Earth and Environmental Science
Ecology
Ecotoxicology
Environment
Environmental Management
Enzymes
Ethylenediaminetetraacetic acid
Fluoranthene
Fluorenes - toxicity
Growth rate
Incubation period
Inoculation
Lipids
Metabolism
Microalgae
Parameters
Peroxidase
Physiological aspects
Phytoplankton
Polycyclic aromatic hydrocarbons
Superoxide dismutase
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Summary:In the present study, the potential of a microalga, Chlorella vulgaris , was assessed for the bioremediation of fluoranthene (FLT), a four ring polycyclic aromatic hydrocarbon (PAH). With an initial cell density of C. vulgaris (OD 680  = 0.100), 54–58% of 25 μM FLT was removed from the growth medium within 3 days and almost 90–94% after 7 days of incubation. Enzymatic studies confirmed that the enzyme involved in FLT metabolism was catechol 2,3, dioxygenase (C2,3D) which increased almost 2 times in 5 μM FLT and 2.4 times in 25 μM FLT inoculated culture. Activity of dehydrogenase and superoxide dismutase (SOD) was significantly reduced, while peroxidase (POD) activity was induced very prominently in FLT inoculated cultures. Changes in growth, physiological parameters and biochemical compositions of the algae with 5 μM and 25 μM FLT were also analyzed and compared to control. The analysis showed that parameters including growth rate, biomass, chlorophyll, carbohydrate and protein contents, were negatively affected by the higher concentration of FLT, whereas the lipid and carotenoids content significantly increased. To our knowledge, this is the first report to suggest the role of C2,3D pathway for the metabolism of FLT in a eukaryotic algae.
ISSN:0963-9292
1573-3017
DOI:10.1007/s10646-020-02334-w