Effects of the prebiotic inulin‐type fructans on post‐antibiotic reconstitution of the gut microbiome

• Published in: Journal of applied microbiology 2021-03, Vol.130 (3), p.634-649
• Main Authors: Tao, C., Zeng, W., Zhang, Q., Liu, G., Wu, F., Shen, H., Zhang, W., Bo, H., Shao, H.
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents; antibiotic cocktail (ABx); Antibiotics; Bacteria - isolation & purification; Colitis; Colitis - chemically induced; Colitis - genetics; Colitis - microbiology; Colon - metabolism; Community composition; Composition; Context; Data recovery; Digestive system; Dysbacteriosis; Fructans; Fructans - pharmacology; Gastrointestinal Microbiome - drug effects; Gastrointestinal tract; Gene expression; gut microbiota; Health promotion; Homeostasis; Homeostasis - drug effects; Intestinal microflora; Intestine; Inulin; inulin‐type fructans (ITFs); Male; Mice; Mice, Inbred BALB C; Microbiomes; Microbiota; Microorganisms; Opportunist infection; prebiotic; Prebiotics; Probiotics; reconstitution; Relative abundance; Spontaneous recovery; Supplements; Transcriptome - drug effects; Transcriptomes
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.14827

Aims Interventions using prebiotic inulin‐type fructans (ITFs) are widely prescribed to modulate the gut microbiota composition and activity to promote health. However, the impacts of ITFs on post‐antibiotic reconstitution of the gut microbiome remain incompletely understood. The aim of the present study was to investigate the effects of ITFs supplementation on intestinal inflammation, the composition of the intestinal microbiota and the colonic transcriptome after antibiotic treatment. Methods and Results Male BALB/c mice were subjected to an antibiotic cocktail (ABx) treatment for 7 days, and their microbiomes were then reconstituted either spontaneously or with ITFs supplementation (5%) for 14 days. Our data showed that ITFs supplementation delayed the recovery of antibiotic‐induced colitis compared with the spontaneous recovery. Neither ITFs supplementation nor spontaneous recovery could restore the microbial community composition at the genus level back to its initial composition. ITFs supplementation increased the relative abundance of some beneficial bacteria and butyrate levels, but resulted in selective blooms of some opportunistic pathogens and elevated the pathways associated with diseases linked to gut microbiota function. Both ITFs supplementation and spontaneous recovery could restore the colonic transcriptome nearly to the initial profile to a certain extent; however, ITFs supplementation delayed the restoration of the immunoglobulin genes compared to spontaneous recovery. Conclusion These data showed that post‐antibiotic ITFs consumption did not always lead to beneficial effects but might lead to potential adverse effects in the context of dysbiosis. Significance and Impact of the Study These findings highlighted that caution is required when supplementing ITFs to restore intestinal homeostasis in the context of dysbiosis resulting from broad‐spectrum antibiotics.