Levan modified DNA biosensor for voltammetric detection of daunorubicin-DNA interaction

• Published in: Sensors and actuators. B, Chemical Chemical, 2021-01, Vol.326, p.128818, Article 128818
• Main Authors: Congur, Gulsah, Eksin, Ece, Erdem, Arzum
• Format: Article
• Language: English
• Subjects: Anticancer properties; Biocompatibility; Biomolecular interaction; Biopolymers; Biosensors; Daunorubicin; Deoxyribonucleic acid; DNA; Electrochemical analysis; Electrochemical biosensor; Levan; Optimization; Voltammetry
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2020.128818

[Display omitted] •LVN modified graphite electrodes were developed as single-use electrochemical DNA biosensor.•Pencil graphite electrode (PGE) was used for levan modification to develop novel biosensor platform.•The voltammetric detection of daunorubicin (DNR), DNA and the interaction of DNR with DNA was determined using LVN-PGE.•The application of LVN modified DNA biosensor to drug-DNA interaction was presented for the first time in our study. Levan (LVN) is a fructan homopolysaccharide biopolymer which has the unique properties for development of a biosensor platform. Herein, LVN modified disposable electrochemical biosensor was developed for detection of DNA, daunorubicin (DNR) and the biomolecular interaction of DNR with DNA. For this purpose, pencil graphite electrodes (PGE) were used as biosensor platform. The microscopic and electrochemical characterization of LVN-PGEs were done in contrast to the control group and the results were showed that the LVN was successfully modified onto the PGE surface. The optimization of experimental conditions such as LVN concentration and DNA concentration was performed. The detection limit was estimated as 2.74 μg/mL using the equation I = 0.05 x CfsDNA+ 3.54 with the R2 = 0.99 for DNA using LVN-PGEs. DNR-DNA interaction was investigated under the optimum conditions. Robust, sensitive, biocompatible and disposable biosensor surface was achieved by modification of PGE surface using LVN biopolymer for electrochemical monitoring of DNA, anticancer agent, DNR as well as DNA-DNR interaction. The single-use electrochemical DNA biosensor modified with LVN is a model for further studies on the development of novel biopolymers based biosensors.