Nuclear abnormalities in umbilical cord blood lymphocytes of newborns from the Ahome and Guasave municipalities in Sinaloa, Mexico

Aim We measured the frequency of nuclear abnormalities of 210 blood samples from the umbilical cord, since human fetuses are exposed to environmental mixtures of pesticides that induce DNA damage. Methods The determinations were made through the micronucleus assay test in lymphocytes from the umbili...

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Bibliographic Details
Published in:The journal of obstetrics and gynaecology research 2021-03, Vol.47 (3), p.968-977
Main Authors: Martínez‐Valenzuela, Carmen, Huichapan, José, Ortega, Luis Daniel, Irineo, Ana Bertha, Zenteno, Edgar, Ruiz‐Ramos, Rubén
Format: Article
Language:English
Subjects:
Chromatin
Cord blood
DNA damage
Fetuses
genotoxic damage
Lymphocytes
Mexico
micronucleus
Mutagens
Neonates
Perfusion
Umbilical cord
umbilical cord blood
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Summary:Aim We measured the frequency of nuclear abnormalities of 210 blood samples from the umbilical cord, since human fetuses are exposed to environmental mixtures of pesticides that induce DNA damage. Methods The determinations were made through the micronucleus assay test in lymphocytes from the umbilical cord blood of newborns whose mothers live in Ahome (n = 105) and Guasave (n = 105), Sinaloa, Mexico. Results The average frequency of anomalies in 1000 cells were, respectively: micronucleus 0.4 vs. 2.9, pyknotic cells 18.3 vs. 109.2, chromatin condensation 7.7 vs. 150.1, karyolitic cells 1.8 vs. 24.4, and binucleated cells 4.9 vs. 74.6. The calculated Pearson correlation factors of nuclear abnormality frequencies between both municipalities were low and negative, suggesting that they did not correlate between the Ahome and Guasave newborns and indicating a higher number of mothers exposed in Guasave. Conclusion Our data suggest that monitoring nuclear abnormalities in umbilical cord blood samples could be a useful tool to identify transplacental mutagens perfusion that is being discharged into the local environment.
ISSN:1341-8076
1447-0756
DOI:10.1111/jog.14624