Expression of CCoAOMT from Zingiber officinale Roscoe under NaCl Stress and Its Regulatory Role in 6-Gingerol Biosynthesis

Ginger ( Zingiber officinale Roscoe) is of high economic value as a medicinal and food resource. 6‑Gingerol is the predominant bioactive component in the rhizome of ginger. A crucial enzyme involved in 6-gingerol biosynthesis is caffeoyl coenzyme A O -methyltransferase (CCoAOMT). In the present stud...

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Bibliographic Details
Published in:Russian journal of plant physiology 2021-03, Vol.68 (2), p.286-292
Main Authors: Tang, J.-M., Liao, Q.-H., Hu, T., Qi, L.-W., Li, Z.-X.
Format: Article
Language:English
Subjects:
Aquatic plants
Biomedical and Life Sciences
Biosynthesis
Coenzyme A
Food resources
Gene silencing
Genetic resources
Ginger
Gingerol
Homology
Life Sciences
Methyltransferase
Plant Physiology
Plant Sciences
Research Papers
Rhizomes
Sodium chloride
Transcription
Zingiber officinale
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Summary:Ginger ( Zingiber officinale Roscoe) is of high economic value as a medicinal and food resource. 6‑Gingerol is the predominant bioactive component in the rhizome of ginger. A crucial enzyme involved in 6-gingerol biosynthesis is caffeoyl coenzyme A O -methyltransferase (CCoAOMT). In the present study, the cDNA sequence of Zo CCoAOMT from ginger was cloned. The expression level of Zo CCoAOMT and the contents of 6-gingerol in the rhizome were analyzed in ginger plants treated with different concentrations of NaCl. The full-length Zo CCoAOMT cDNA consisted of 738 bp and showed 96.34% sequence homology with Curcuma longa CCoAOMT . Transcription of Zo CCoAOMT was highly consistent with the trend in accumulation of 6-gingerol. The Zo CCoAOMT transcript level and 6-gingerol content in the rhizome were highest under treatment with 25 g/L NaCl but decreased under higher concentrations of NaCl, which suggested a positive regulatory relationship between Zo CCoAOMT and 6-gingerol synthesis. Virus-induced gene silencing (VIGS) analysis further verified the positive regulatory role of ZoCCoAOMT in 6-gingerol synthesis. The results provide fundamental information for clarification of the role of Zo CCoAOMT in the 6-gingerol biosynthesis pathway of Z . officinale , and also gene resources for 6-gingerol biosynthesis.
ISSN:1021-4437
1608-3407
DOI:10.1134/S1021443721020199