Nuclear hyperpolarization of (1-C)-pyruvate in aqueous solution by proton-relayed side-arm hydrogenation

We employ Parahydrogen Induced Polarization with Side-Arm Hydrogenation (PHIP-SAH) to polarize (1- 13 C)-pyruvate. We introduce a new method called proton-relayed side-arm hydrogenation (PR-SAH) in which an intermediate proton is used to transfer polarization from the side-arm to the 13 C-labelled s...

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Bibliographic Details
Published in:Analyst (London) 2021-03, Vol.146 (5), p.1772-1778
Main Authors: Dagys, Laurynas, Jagtap, Anil P, Korchak, Sergey, Mamone, Salvatore, Saul, Philip, Levitt, Malcolm H, Glöggler, Stefan
Format: Article
Language:English
Subjects:
Aqueous solutions
Hydrogenation
Induced polarization
Metabolites
NMR
Nuclear magnetic resonance
Protons
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Summary:We employ Parahydrogen Induced Polarization with Side-Arm Hydrogenation (PHIP-SAH) to polarize (1- 13 C)-pyruvate. We introduce a new method called proton-relayed side-arm hydrogenation (PR-SAH) in which an intermediate proton is used to transfer polarization from the side-arm to the 13 C-labelled site of the pyruvate before hydrolysis. This significantly reduces the cost and effort needed to prepare the precursor for radio-frequency transfer experiments while still maintaining acceptable polarization transfer efficiency. Experimentally we have attained on average 4.33% 13 C polarization in an aqueous solution of (1- 13 C)-pyruvate after about 10 seconds of cleavage and extraction. PR-SAH is a promising pulsed NMR method for hyperpolarizing 13 C-labelled metabolites in solution, conducted entirely in high magnetic field. We employ parahydrogen induced polarization with side-Arm hydrogenation (PHIP-SAH) to polarize (1- 13 C)-pyruvate via a new pulsed magnetic resonance experiment.
ISSN:0003-2654
1364-5528
DOI:10.1039/d0an02389b