Solid-state colorimetric polydiacetylene liposome biosensor sensitized by gold nanoparticles

Polydiacetylene (PDA), a conjugated polymer, has attracted attention for realization of a label-free real-time colorimetric biosensor because it exhibits large and rapid colorimetric responses upon the binding of biomolecules. This is due to the conformational distortion of its conjugated backbone....

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Bibliographic Details
Published in:Analyst (London) 2021-03, Vol.146 (5), p.1682-1688
Main Authors: Kim, Jayoung, Moon, Byeong-Seok, Hwang, Eunhee, Shaban, Samy, Lee, Wonjung, Pyun, Do-Gi, Lee, Dong Hyun, Kim, Dong-Hwan
Format: Article
Language:English
Subjects:
Aptamers, Nucleotide
Backbone
Binding
Biomolecules
Biosensing Techniques
Biosensors
Colorimetry
Distortion
Gold
Liposomes
Metal Nanoparticles
Nanoparticles
Polyacetylene Polymer
Polydiacetylenes
Sensitivity enhancement
Solid state
Substrates
Thrombin
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Summary:Polydiacetylene (PDA), a conjugated polymer, has attracted attention for realization of a label-free real-time colorimetric biosensor because it exhibits large and rapid colorimetric responses upon the binding of biomolecules. This is due to the conformational distortion of its conjugated backbone. However, solid-state PDA biosensors for point-of-care diagnosis remain unexplored. We describe a highly sensitive solid-state biosensor based on PDA liposomes. We employed gold nanoparticles (AuNPs) on PDA liposomes as the molecular-binding-signal sensitizer, which provides additional conformational distortion in the backbone structure of PDA by exerting steric repulsion to the attached biomolecules. To prove the concept, AuNPs and a thrombin-binding-aptamer were individually functionalized on PDA liposomes, which were attached to a substrate for the detection of thrombin. We found that the sensitivity was enhanced 2.5 times in the presence of AuNPs compared with the case without AuNPs. Because the steric repulsion of the AuNPs is target-independent, we believe that our solid-state biosensor provides a path toward advanced solid-state biosensors. Solid-state polydiacetylene liposome biosensors are sensitized by gold nanoparticles to exhibit clear colorimetric response from blue to red upon biomolecular binding. This hybrid system will pave the way for portable colorimetric biosensors.
ISSN:0003-2654
1364-5528
DOI:10.1039/d0an02375b