Aerobic degradation of 4-fluoroaniline and 2,4-difluoroaniline: performance and microbial community in response to the inocula

• Published in: Biodegradation (Dordrecht) 2021-02, Vol.32 (1), p.53-71
• Main Authors: Zhao, Zhi-Qing, Wei, Xiao-Meng, Shen, Xiao-Li, Abbas, Ghulam, Fan, Rui, Jin, Yi
• Format: Article
• Language: English
• Subjects: Acclimation; Acclimatization; Aerobic conditions; Analysis; Aniline Compounds; Aquatic Pollution; Batch reactors; Biodegradation; Biodegradation, Environmental; Biomedical and Life Sciences; Bioreactors; Consortia; Degradation; Drug resistance in microorganisms; Enrichment; Geochemistry; Industrial parks; Inoculum; Life Sciences; Medical wastes; Microbiology; Microbiota; Microorganisms; Municipal wastes; Municipal wastewater; Next-generation sequencing; Original Paper; Pharmaceutical industry wastes; Recovery time; Sequencing; Sewage; Shock resistance; Similarity; Soil Science & Conservation; Substrates; Terrestrial Pollution; Waste Management/Waste Technology; Waste Water Technology; Wastewater; Wastewater treatment; Water Management; Water Pollution Control
• ISSN: 0923-9820; 1572-9729
• DOI: 10.1007/s10532-021-09925-2

In this study, a distinct inoculum was investigated as an isolated variable within sequencing batch reactors via a comparison of the 4-fluoroaniline (4-FA) or 2,4-difluoroaniline (2,4-DFA) removal amounts. The inocula were derived from a treatment plant for treating pharmaceutical wastewater plus a small amount of municipal sewage (PMS), a treatment plant for treating fluoridated hydrocarbon wastewater (FHS), and a treatment plant for treating the comprehensive wastewater in an industrial park (CIS). There were slight differences among the degradation patterns of the 4-FA for the three inocula, whether during the enrichment period or the high concentration shock period. In contrast, it was observed that the degradation efficiency of 2,4-DFA initially varied with the inocula. The FHS-derived inoculum was determined to be optimal, exhibiting the earliest degradation reaction only after an acclimation of 7 days had the highest degradation rate constant of 0.519 h −1 , and had the fastest recovery time of three weeks after high concentration shock. Additionally, compared with the PMS-derived inoculum, the CIS-derived inoculum exhibited an earlier degradation reaction within three weeks, and a higher microbial diversity, but a lower shock resistance and degradation rate constant of 0.257 h −1 . High-throughput sequencing demonstrated that each final consortium was different in composition, and the microbial consortia developed well on the inoculum and substrate. In comparison of the similarity among the three 2,4-DFA enrichment cultures, the higher similarity (63.9–70.0%) among three final consortia enriching with 4-FA was observed. The results indicated that the inoculum played an important role in the degradation of FAs and the microbial bacterial communities of final consortia, and the effect extent might well depend on the fluorinated level of FAs.