Microwave Sensing of Yeast Cell Species and Viability

We report the development of a simple interferometer-based microwave sensing system for multiple frequency characterization and differentiation of in-flow yeast cells. The interferometer uses a simple microstrip line, integrated with a microfluidic channel, for single-cell measurement. An algorithm...

Full description

Saved in:
Bibliographic Details
Published in:IEEE transactions on microwave theory and techniques 2021-03, Vol.69 (3), p.1875-1886
Main Authors: Osterberg, Jeffrey A., Dahal, Neelima, Divan, Ralu, Miller, Christina S., Moline, David, Caldwell, Thomas P., Yu, Xianzhong, Harcum, Sarah W., Wang, Pingshan
Format: Article
Language:English
Subjects:
Algorithms
BASIC BIOLOGICAL SCIENCES
Brewing yeast
Broadband communication
Complex permittivity
cytometry
Diameters
Differentiation (biology)
Frequency measurement
Microfluidics
Microstrip
Microstrip transmission lines
Microwave measurement
microwave sensing
Permittivity
Permittivity measurement
Polystyrene resins
Prediction models
Sensors
single cell analysis
Yeast
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We report the development of a simple interferometer-based microwave sensing system for multiple frequency characterization and differentiation of in-flow yeast cells. The interferometer uses a simple microstrip line, integrated with a microfluidic channel, for single-cell measurement. An algorithm was developed and verified with high-frequency structure simulator (HFSS) for complex permittivity, \varepsilon ^{\ast }(f) = \varepsilon '(f)- j\varepsilon ''(f) , extraction from measured scattering parameters. The sensing system and the algorithm were evaluated by measuring polystyrene particles of different diameters and at different interferometer operating frequencies. Viable and nonviable Saccharomyces cerevisiae and Saccharomyces pastorianus cells were measured at those frequencies. The results showed frequency-dependent permittivity values for each species of yeast and viability as well as frequency-dependent permittivity differences between different yeast types. The differences at some frequency points are significant and enable the differentiation of cells in mixed suspension, which is also demonstrated with a prediction model developed in this work.
ISSN:0018-9480
1557-9670
DOI:10.1109/TMTT.2020.3048176