Fast Blocking Effect of Gallamine on LCC Channels: Analysis Using a Truncated Kinetic Scheme

Modulation of Large-conductance cationic (LCC) channel currents through the inner nuclear membrane of rat Purkinje neurons by gallamine was estimated using analysis of the amplitude histograms. A kinetic scheme consisting of one open state and one blocked state of the channel was used. The search fo...

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Bibliographic Details
Published in:Neurophysiology (New York) 2020-07, Vol.52 (4), p.250-255
Main Authors: Lunko, O. V., Lunko, O. O.
Format: Article
Language:English
Subjects:
Analysis
Biomedical and Life Sciences
Biomedicine
Channel gating
Conductance
Neurophysiology
Neurosciences
Purkinje cells
Tubocurarine
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Summary:Modulation of Large-conductance cationic (LCC) channel currents through the inner nuclear membrane of rat Purkinje neurons by gallamine was estimated using analysis of the amplitude histograms. A kinetic scheme consisting of one open state and one blocked state of the channel was used. The search for optimal kinetic parameters of the truncated model was performed for the analysis of the blocking effect. The estimated rate constants of blocking and unblocking by 100 μM gallamine at –40 mV were 17880 ± ± 975 and 12790 ± 922, respectively. The obtained data show that gallamine is a fast blocker of the above channels; blocking is accompanied by ion current fluctuations suitable for the analysis using the two-state model if contribution of the events formed by intrinsic ion channel activity can be neglected.
ISSN:0090-2977
1573-9007
DOI:10.1007/s11062-021-09879-4