Peanut (Arachis hypogaea L.) S‐adenosylmethionine decarboxylase confers transgenic tobacco with elevated tolerance to salt stress

• Published in: Plant biology (Stuttgart, Germany) Germany), 2021-03, Vol.23 (2), p.341-350
• Main Authors: Meng, D.‐Y., Yang, S., Xing, J.‐Y., Ma, N.‐N., Wang, B.‐Z., Qiu, F.‐T., Guo, F., Meng, J., Zhang, J.‐l., Wan, S.‐B., Li, X.‐G., Luo, Z.‐B.
• Format: Article
• Language: English
• Subjects: Abiotic stress; Adenosylmethionine; Adenosylmethionine decarboxylase; Adenosylmethionine Decarboxylase - genetics; Adenosylmethionine Decarboxylase - metabolism; Antioxidants; Arachis - enzymology; Arachis - genetics; Arachis hypogaea; Damage; Enzymatic activity; Enzyme activity; Gene Expression Regulation, Plant; Germination; Leaves; Membranes; Nicotiana - drug effects; Nicotiana - enzymology; Nicotiana - genetics; Peanut (Arachis hypogaea L.); Peanuts; Phylogeny; Physiological effects; Plant resistance; Plants, Genetically Modified - drug effects; Plants, Genetically Modified - enzymology; Plants, Genetically Modified - genetics; Polyamines; Proline; Reactive oxygen species; S-Adenosylmethionine; Salinity tolerance; salt stress; Salt Stress - genetics; Salts; SAMDC; Seedlings; Sodium chloride; Sodium Chloride - toxicity; Spermidine; Spermine; Stress response; Tobacco; Transcription; Transgenic plants; transgenic tobacco
• ISSN: 1435-8603; 1438-8677
• DOI: 10.1111/plb.13173

Polyamines play an important role in stress response. In the pathway of polyamines synthesis, S-adenosylmethionine decarboxylase (SAMDC) is one of the key enzymes. In this study, a full length cDNA of SAMDC (AhSAMDC) was isolated from peanut (Arachis hypogaea L.). Phylogenetic analysis revealed high sequence similarity between AhSAMDC and SAMDC from other plants. In peanut seedlings exposed to sodium chloride (NaCl), the transcript level of AhSAMDC in roots was the highest at 24 h that decreased sharply at 72 and 96 h after 150 mM NaCl treatment. However, the expression of AhSAMDC in peanut leaves was significantly inhibited, and the transcript levels in leaves were not different compared with control These results implied the tissue‐specific and time-specific expression of AhSAMDC. The physiological effects and functional mechanism of AhSAMDC were further evaluated by overexpressing AhSAMDC in tobaccos. The transgenic tobacco lines exhibited higher germination rate and longer root length under salt stress. Reduced membrane damage, higher antioxidant enzyme activity, and higher proline content were also observed in the transgenic tobacco seedlings. What's more, AhSAMDC also led to higher contents of spermidine and spermine, which can help to scavenge reactive oxygen species. Together, this study suggests that AhSAMDC enhances plant resistance to salt stress by improving polyamine content and alleviating membrane damage AhSAMDC subcloned into tobacco plants may play an important role in alleviating oxidative stress and membrane damages by improving antioxidant enzymes under salt stress.