A RT-LAMP based hydrogen ion selective electrode sensing for effective detection HIV-1 RNA with high-sensitivity

• Published in: Sensors and actuators. B, Chemical Chemical, 2021-02, Vol.329, p.129118, Article 129118
• Main Authors: Kong, Hui, Zhang, Wei, Yao, Jia, Li, Chao, Lu, Renfei, Guo, Zhen, Li, Jinze, Li, Chuanyu, Li, Yingxue, Zhang, Chiyu, Zhou, Lianqun
• Format: Article
• Language: English
• Subjects: Carbon nanotubes; Graphene; H+-selective solid electrode; HIV-1; Hydrogen; Hydrogen ion concentration; Hydrogen ions; Ion concentration; Point-of-care; RT-LAMP; Screen-printed graphene electrode; Sensitivity; Sensors; Solid electrolytes
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2020.129118

[Display omitted] •The RT-LAMP products using a solid-state hydrogen ion selective electrode sensor.•H+-RT-LAMP sensor stability was improved and sensitivity was 63.567 mV/pH.•The H+-RT-LAMP sensor was used to detect the HIV-1 RNA clinical sample.•Portable platform for the lowest detection limit of 10 copies/tube within 25 min. Development of a simple, sensitive and specific method for HIV-1 assay is demanded. In some emergency situations, a portable reverse transcription loop-mediated isothermal amplification (RT-LAMP) platform is necessary. A novel hydrogen ion-selective RT-LAMP (H+-RT-LAMP) sensor was developed for highly sensitive HIV-1 using hydrogen ion concentration as a signal producing compound. The H+-RT-LAMP sensor was based on screen-printed with carbon-doped graphene conductive layer, carbon nanotubes (CNTs) solid electrolyte layer, and reference electrode KCl functional layer, which provided superior electrical signals, high sensitivity and stability for HIV-1 detection. The rate of hydrogen ion production was related to the viral load (VL) during the amplification of HIV-1. The sensitive HIV-1 RNA detection range was achieved from 0 copies/μL to 105 copies/μL. The proposed H+-RT-LAMP sensor was applied to detect HIV-1 RNA in real human serum. 57 samples were detected and satisfactory results were obtained. Compared with commercial instruments, the detection results of H+-RT-LAMP sensor demonstrated a perfect concordance rate of 96.5 %, and the limit of detection (LOD) for the clinical samples was 10 copies per tube. Time to get the result is from 10 min to 25 min. Therefore, the proposed H+-RT-LAMP sensor was particularly suitable as a point-of-care device for the on-site detection of HIV-1.