A Microbial Consortium Removing Phosphates under Conditions of Cyclic Aerobic-Anaerobic Cultivation

— Formation of a community of phosphate-accumulating microorganisms in a laboratory sequential batch reactor (SBR) ensuring alternated aerobic and anaerobic conditions during periodic removal and addition of the medium were investigated. The bioreactor removed 50% phosphorus from the incoming medium...

Full description

Saved in:
Bibliographic Details
Published in:Microbiology (New York) 2021, Vol.90 (1), p.66-77
Main Authors: Pelevina, A. V., Berestovskaya, Yu. Yu, Grachev, V. A., Dorofeeva, I. K., Sorokin, V. V., Dorofeev, A. G., Kallistova, A. Yu, Nikolaev, Yu. A., Kotlyarov, R. Yu, Beletskii, A. V., Ravin, N. V., Pimenov, N. V., Mardanov, A. V.
Format: Article
Language:English
Subjects:
Aerobic microorganisms
Anaerobic conditions
Anaerobic microorganisms
Bacteroidetes
Biomedical and Life Sciences
Bioreactors
Candidatus Accumulibacter
Community composition
Cytology
Dechloromonas
Experimental Articles
Heterotrophs
Life Sciences
Medical Microbiology
Microbiology
Next-generation sequencing
Phosphorus
Proteobacteria
rRNA 16S
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:— Formation of a community of phosphate-accumulating microorganisms in a laboratory sequential batch reactor (SBR) ensuring alternated aerobic and anaerobic conditions during periodic removal and addition of the medium were investigated. The bioreactor removed 50% phosphorus from the incoming medium after 22 days from the start-up. Microscopy and X-ray microassay revealed the of cells of diverse morphology that contained phosphorus-enriched granules. High-throughput sequencing of the 16S rRNA gene fragments carried out on days 0, 8, 15, and 22 showed changes in the community composition and its decreasing diversity. On day 22, approximately twofold increase of the relative abundances of Bacteroidetes (up to 43% of the 16S rRNA gene sequences) and Proteobacteria of the classes alpha (up to 15%) and beta (up to 27%) was observed. While at the onset of the reactor operation, typical PAOs related to “ Candidatus  Accumulibacter” (class Betaproteobacteria ) constituted 0.2% of the community, they were not detected on day 22. The most likely PAO candidates were beta-proteobacteria of the genus Dechloromonas , the share of which increased from 0.7 to 11% by the time of the highest phosphorus removal from the inflowing medium. The relative abundance of heterotrophs of the genus Zoogloea (family Rhodocyclaceae ) increased from 0.1 to 11.5%.
ISSN:0026-2617
1608-3237
DOI:10.1134/S0026261721010082