Fabrication of Miniaturized Microfluidic Paper-Based Analytical Devices for Sandwich Enzyme-Linked Immunosorbent Assays using INKJET Printing

Main application of microfluidic paper-based analytical devices (µPADs) is for low-cost and portable assays. In order to reduce the cost, miniaturization seems to be an effective way to save reagents, samples, materials, etc. Present study demonstrates that mini-µPADs based on nitrocellulose membran...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2021-03, Vol.57 (2), p.257-261
Main Authors: Le, N. N., Phan, H. C. T., Dang, D. M. T., Dang, C. M.
Format: Article
Language:English
Subjects:
Assaying
Biochemistry
Biomedical and Life Sciences
Cellulose esters
Cellulose nitrate
Chorionic gonadotropin
Colorimetry
Cost analysis
Digital cameras
Enzyme-linked immunosorbent assay
Enzymes
Fabrication
Gonadotropins
Hydrophobicity
Immunoassays
Inkjet printing
Life Sciences
Mathematical analysis
Medical Microbiology
Microbiology
Microfluidics
Miniaturization
Pituitary (anterior)
Portable equipment
Reagents
Signal processing
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Summary:Main application of microfluidic paper-based analytical devices (µPADs) is for low-cost and portable assays. In order to reduce the cost, miniaturization seems to be an effective way to save reagents, samples, materials, etc. Present study demonstrates that mini-µPADs based on nitrocellulose membrane with only 2 main channels can perform sandwich enzyme-linked immunosorbent assays (ELISA) for quantitative determination of human chorionic gonadotropin (hCG). Moreover, the width and the length of these devices are only about 4.0 and 33.4 mm, respectively. Inkjet printing technology, having the advantages of simple and material-saving process, was used to create hydrophobic barrier. Colorimetric signals from ELISA captured by digital camera and measured by ImageJ software shows that these µPADs can quantitative determine of hCG in the range from 5 to 10 000 ng/mL. Furthermore, duration for each tests is only about 5 min. The success in fabricating miniaturized µPADs shows the potentials to reduce the size of µPADs as well as the amount of needed samples, time and reagents. For all these reasons, this miniaturization concept can be applied to many further applications of analytical biochemical assays.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683821020071