Occurrence and characterization of virus species associated with black pepper (piper nigrum l.) virus diseases in Hainan province, China

Black pepper (Piper nigrum L.) is one of the most ancient spice crops in the world. Hainan Province is the largest black pepper‐producing region in China. Viral disease is one of the main factors affecting black pepper. However, there are rarely studies on black pepper viral disease in China. We per...

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Bibliographic Details
Published in:Journal of phytopathology 2021-04, Vol.169 (4), p.247-252
Main Authors: Che, Haiyan, Cao, Xueren, Liu, Peipei, Luo, Daquan
Format: Article
Language:English
Subjects:
Deoxyribonucleic acid
DNA
DNA sequencing
Genomes
Nucleotide sequence
phylogenetic analysis
Phylogeny
Piper nigrum
Piper nigrum c.v. Reyin‐1
piper yellow mottle virus
Plant virus diseases
RT‐PCR
Signs and symptoms
Spices
Viral diseases
Viruses
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Summary:Black pepper (Piper nigrum L.) is one of the most ancient spice crops in the world. Hainan Province is the largest black pepper‐producing region in China. Viral disease is one of the main factors affecting black pepper. However, there are rarely studies on black pepper viral disease in China. We performed a survey in five major black pepper‐growing counties (Qionghai, Wenchang, Wanning, Haikou and Ding'an) of Hainan Province and collected 589 samples, with virus‐like symptoms from 2014 to 2019. Various viruses were identified including cucumber mosaic virus (CMV), piper yellow mottle virus (PYMoV), Piper DNA virus 1 (PDV‐1) and Piper DNA virus 2 (PDV‐2) using small RNA deep sequencing. The RT‐PCR screening of field samples indicated three types of infection: CMV alone (5.56%), PYMoV + PDV‐1 + PDV‐2 (25.00%) and PYMoV + CMV +PDV‐1 + PDV‐2 (69.44%). The data suggested that PYMoV might be the main pathogen causing virus diseases of black pepper. Two complete genome sequences of PYMoV were obtained. Phylogenetic analysis showed that PYMoV isolates from China were placed in a branch distinct from PYMoV isolates from India. To our knowledge, this is the first report of PYMoV, PDV‐1 and PDV‐2 naturally infecting black pepper, and the first genome sequence of PYMoV in China.
ISSN:0931-1785
1439-0434
DOI:10.1111/jph.12981