Exogenous 24‐epibrassinolide activates detoxification enzymes to promote degradation of boscalid in cherry tomatoes

• Published in: Journal of the science of food and agriculture 2021-04, Vol.101 (6), p.2210-2217
• Main Authors: Yang, Yajie, Mao, Linchun, Guan, Weiliang, Wei, Xiaobo, Shao, Yelin, Luo, Zisheng, Lin, Xingyu, Li, Li
• Format: Article
• Language: English
• Subjects: 24‐epibrassinolide; Biphenyl Compounds - chemistry; Biphenyl Compounds - metabolism; boscalid; Brassinosteroids - pharmacology; cherry tomato; Degradation; Detoxification; distribution; Enzyme Activation - drug effects; Enzymes; Food safety; Fruit - chemistry; Fruit - drug effects; Fruit - enzymology; Fruit - metabolism; Fruits; Fungicides, Industrial - chemistry; Fungicides, Industrial - metabolism; Glutathione; Glutathione reductase; Glutathione Reductase - metabolism; Glutathione Transferase - metabolism; Lycopersicon esculentum - chemistry; Lycopersicon esculentum - drug effects; Lycopersicon esculentum - enzymology; Lycopersicon esculentum - metabolism; Niacinamide - analogs & derivatives; Niacinamide - chemistry; Niacinamide - metabolism; Peroxidase; Peroxidase - metabolism; Pesticide residues; Pesticide Residues - chemistry; Pesticide Residues - metabolism; Pesticides; Plant Proteins - metabolism; Pretreatment; Pulp; Reductases; Residues; Spatial distribution; Steroids, Heterocyclic - pharmacology; Tomatoes
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.10840

BACKGROUND Boscalid is often used to extend the storage time of postharvest cherry tomato. Pesticide residue has become an issue of food safety. This study sought to investigate the spatial distribution of boscalid residue in cherry tomato fruits and to determine the effect of 24‐epibrassinolide (EBR) in promoting boscalid degradation. RESULTS Boscalid could quickly penetrate into cherry tomatoes, but mainly remained in the peel. The migration of boscalid from the peel into the core was a time‐consuming and complex process during storage. After 72 h, boscalid residues in the pulp and the core began to accumulate gradually. The exogenous application of EBR activated peroxidase, glutathione reductase and glutathione S‐transferase, and effectively promoted the degradation of boscalid by a maximum decrease of 44.8% in peel, 54.0% in pulp and 71.2% in core. CONCLUSION As one of the common pesticides, boscalid had a strong ability to enter the cherry tomato and thus become a potential risk for public consumption. Therefore, rational use of pesticides is recommended. The results of this study indicate that the possible risk of boscalid residue could be alleviated by EBR pretreatment through activating detoxification enzymes. © 2020 Society of Chemical Industry