Gallocyanine as a Fluorogen for the Identification of NADPH-Dependent Production of Superoxide Anion Radical by Blood Cells

— The interaction of the oxazine dye gallocyanine with reactive oxygen ( Н 2 О 2 ) and halogen (HOCl) species has been studied by spectrophotometry, mass spectrometry, and spectrofluorimetry. It has been shown that gallocyanine reacts with HOCl and (but not with Н 2 О 2 ) to form fluorescent product...

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Bibliographic Details
Published in:Russian journal of bioorganic chemistry 2021, Vol.47 (1), p.299-306
Main Authors: Panasenko, O. M., Reut, V. E., Borodina, I. V., Matyushkina, D. S., Ivanov, V. A., Grigorieva, D. V., Gorudko, I. V., Sokolov, A. V., Cherenkevich, S. N.
Format: Article
Language:English
Subjects:
Anions
Antioxidants
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Blood cells
Chemical sensors
Chemoreceptors
Fluorescence
Life Sciences
Mass spectrometry
Neutrophils
Organic Chemistry
Oxidation
Spectrofluorimetry
Spectrophotometry
Xanthine oxidase
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Summary:— The interaction of the oxazine dye gallocyanine with reactive oxygen ( Н 2 О 2 ) and halogen (HOCl) species has been studied by spectrophotometry, mass spectrometry, and spectrofluorimetry. It has been shown that gallocyanine reacts with HOCl and (but not with Н 2 О 2 ) to form fluorescent products. It has been found using the inhibition assay that, both in the xanthine/xanthine oxidase system and in activated human blood neutrophils, the main contribution to the conversion of gallocyanine to a fluorophore comes from its reaction with The results obtained suggest that gallocyanine can act as a fluorogenic chemosensor and be used for estimating the activation of neutrophils and the NADPH-dependent production of superoxide anion radical by neutrophils and other blood cells, as well as for testing antioxidant drugs designed with the aim to correct diseases associated with oxidative stress.
ISSN:1068-1620
1608-330X
DOI:10.1134/S1068162021010179