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Gallocyanine as a Fluorogen for the Identification of NADPH-Dependent Production of Superoxide Anion Radical by Blood Cells
— The interaction of the oxazine dye gallocyanine with reactive oxygen ( Н 2 О 2 ) and halogen (HOCl) species has been studied by spectrophotometry, mass spectrometry, and spectrofluorimetry. It has been shown that gallocyanine reacts with HOCl and (but not with Н 2 О 2 ) to form fluorescent product...
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| Published in: | Russian journal of bioorganic chemistry 2021, Vol.47 (1), p.299-306 |
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| Main Authors: | , , , , , , , , |
| Format: | Article |
| Language: | English |
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| container_end_page | 306 |
| container_issue | 1 |
| container_start_page | 299 |
| container_title | Russian journal of bioorganic chemistry |
| container_volume | 47 |
| creator | Panasenko, O. M. Reut, V. E. Borodina, I. V. Matyushkina, D. S. Ivanov, V. A. Grigorieva, D. V. Gorudko, I. V. Sokolov, A. V. Cherenkevich, S. N. |
| description | —
The interaction of the oxazine dye gallocyanine with reactive oxygen (
Н
2
О
2
) and halogen (HOCl) species has been studied by spectrophotometry, mass spectrometry, and spectrofluorimetry. It has been shown that gallocyanine reacts with HOCl and
(but not with Н
2
О
2
) to form fluorescent products. It has been found using the inhibition assay that, both in the xanthine/xanthine oxidase system and in activated human blood neutrophils, the main contribution to the conversion of gallocyanine to a fluorophore comes from its reaction with
The results obtained suggest that gallocyanine can act as a fluorogenic chemosensor and be used for estimating the activation of neutrophils and the NADPH-dependent production of superoxide anion radical by neutrophils and other blood cells, as well as for testing antioxidant drugs designed with the aim to correct diseases associated with oxidative stress. |
| doi_str_mv | 10.1134/S1068162021010179 |
| format | article |
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The interaction of the oxazine dye gallocyanine with reactive oxygen (
Н
2
О
2
) and halogen (HOCl) species has been studied by spectrophotometry, mass spectrometry, and spectrofluorimetry. It has been shown that gallocyanine reacts with HOCl and
(but not with Н
2
О
2
) to form fluorescent products. It has been found using the inhibition assay that, both in the xanthine/xanthine oxidase system and in activated human blood neutrophils, the main contribution to the conversion of gallocyanine to a fluorophore comes from its reaction with
The results obtained suggest that gallocyanine can act as a fluorogenic chemosensor and be used for estimating the activation of neutrophils and the NADPH-dependent production of superoxide anion radical by neutrophils and other blood cells, as well as for testing antioxidant drugs designed with the aim to correct diseases associated with oxidative stress.</description><identifier>ISSN: 1068-1620</identifier><identifier>EISSN: 1608-330X</identifier><identifier>DOI: 10.1134/S1068162021010179</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Anions ; Antioxidants ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Bioorganic Chemistry ; Blood cells ; Chemical sensors ; Chemoreceptors ; Fluorescence ; Life Sciences ; Mass spectrometry ; Neutrophils ; Organic Chemistry ; Oxidation ; Spectrofluorimetry ; Spectrophotometry ; Xanthine oxidase</subject><ispartof>Russian journal of bioorganic chemistry, 2021, Vol.47 (1), p.299-306</ispartof><rights>Pleiades Publishing, Ltd. 2021. ISSN 1068-1620, Russian Journal of Bioorganic Chemistry, 2021, Vol. 47, No. 1, pp. 299–306. © Pleiades Publishing, Ltd., 2021. Russian Text © The Author(s), 2021, published in Bioorganicheskaya Khimiya, 2021, Vol. 47, No. 1, pp. 153–161.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c311t-f48d2d7469144ac8c4a8ef4f55b473e1bba267c4d548126a9c6ed1e35bc024f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Panasenko, O. M.</creatorcontrib><creatorcontrib>Reut, V. E.</creatorcontrib><creatorcontrib>Borodina, I. V.</creatorcontrib><creatorcontrib>Matyushkina, D. S.</creatorcontrib><creatorcontrib>Ivanov, V. A.</creatorcontrib><creatorcontrib>Grigorieva, D. V.</creatorcontrib><creatorcontrib>Gorudko, I. V.</creatorcontrib><creatorcontrib>Sokolov, A. V.</creatorcontrib><creatorcontrib>Cherenkevich, S. N.</creatorcontrib><title>Gallocyanine as a Fluorogen for the Identification of NADPH-Dependent Production of Superoxide Anion Radical by Blood Cells</title><title>Russian journal of bioorganic chemistry</title><addtitle>Russ J Bioorg Chem</addtitle><description>—
The interaction of the oxazine dye gallocyanine with reactive oxygen (
Н
2
О
2
) and halogen (HOCl) species has been studied by spectrophotometry, mass spectrometry, and spectrofluorimetry. It has been shown that gallocyanine reacts with HOCl and
(but not with Н
2
О
2
) to form fluorescent products. It has been found using the inhibition assay that, both in the xanthine/xanthine oxidase system and in activated human blood neutrophils, the main contribution to the conversion of gallocyanine to a fluorophore comes from its reaction with
The results obtained suggest that gallocyanine can act as a fluorogenic chemosensor and be used for estimating the activation of neutrophils and the NADPH-dependent production of superoxide anion radical by neutrophils and other blood cells, as well as for testing antioxidant drugs designed with the aim to correct diseases associated with oxidative stress.</description><subject>Anions</subject><subject>Antioxidants</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bioorganic Chemistry</subject><subject>Blood cells</subject><subject>Chemical sensors</subject><subject>Chemoreceptors</subject><subject>Fluorescence</subject><subject>Life Sciences</subject><subject>Mass spectrometry</subject><subject>Neutrophils</subject><subject>Organic Chemistry</subject><subject>Oxidation</subject><subject>Spectrofluorimetry</subject><subject>Spectrophotometry</subject><subject>Xanthine oxidase</subject><issn>1068-1620</issn><issn>1608-330X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kE1Lw0AQhoMoWKs_wNuC5-h-JU2OtbUfULTYHryFze5sTYm7cTcBi3_eDVU8iMxhhnnf5x2YKLom-JYQxu82BKcZSSmmBIca5SfRgKQ4ixnDL6dhDnLc6-fRhfd7HEw4yQbR51zUtZUHYSoDSHgk0KzurLM7MEhbh9pXQEsFpq10JUVbWYOsRo_j6XoRT6EB02to7azq5I-66Rpw9qNSgMam3z0LFeAalQd0X1ur0ATq2l9GZ1rUHq6--zDazh62k0W8epovJ-NVLBkhbax5pqga8TQnnAuZSS4y0FwnSclHDEhZCpqOJFcJzwhNRS5TUARYUkpMuWbD6OYY2zj73oFvi73tnAkXC5pgRvM0z5PgIkeXdNZ7B7poXPUm3KEguOhfXPx5cWDokfHBa3bgfpP_h74Ar7h9oQ</recordid><startdate>2021</startdate><enddate>2021</enddate><creator>Panasenko, O. M.</creator><creator>Reut, V. E.</creator><creator>Borodina, I. V.</creator><creator>Matyushkina, D. S.</creator><creator>Ivanov, V. A.</creator><creator>Grigorieva, D. V.</creator><creator>Gorudko, I. V.</creator><creator>Sokolov, A. V.</creator><creator>Cherenkevich, S. N.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2021</creationdate><title>Gallocyanine as a Fluorogen for the Identification of NADPH-Dependent Production of Superoxide Anion Radical by Blood Cells</title><author>Panasenko, O. M. ; Reut, V. E. ; Borodina, I. V. ; Matyushkina, D. S. ; Ivanov, V. A. ; Grigorieva, D. V. ; Gorudko, I. V. ; Sokolov, A. V. ; Cherenkevich, S. N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c311t-f48d2d7469144ac8c4a8ef4f55b473e1bba267c4d548126a9c6ed1e35bc024f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Anions</topic><topic>Antioxidants</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bioorganic Chemistry</topic><topic>Blood cells</topic><topic>Chemical sensors</topic><topic>Chemoreceptors</topic><topic>Fluorescence</topic><topic>Life Sciences</topic><topic>Mass spectrometry</topic><topic>Neutrophils</topic><topic>Organic Chemistry</topic><topic>Oxidation</topic><topic>Spectrofluorimetry</topic><topic>Spectrophotometry</topic><topic>Xanthine oxidase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Panasenko, O. M.</creatorcontrib><creatorcontrib>Reut, V. E.</creatorcontrib><creatorcontrib>Borodina, I. V.</creatorcontrib><creatorcontrib>Matyushkina, D. S.</creatorcontrib><creatorcontrib>Ivanov, V. A.</creatorcontrib><creatorcontrib>Grigorieva, D. V.</creatorcontrib><creatorcontrib>Gorudko, I. V.</creatorcontrib><creatorcontrib>Sokolov, A. V.</creatorcontrib><creatorcontrib>Cherenkevich, S. N.</creatorcontrib><collection>CrossRef</collection><jtitle>Russian journal of bioorganic chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Panasenko, O. M.</au><au>Reut, V. E.</au><au>Borodina, I. V.</au><au>Matyushkina, D. S.</au><au>Ivanov, V. A.</au><au>Grigorieva, D. V.</au><au>Gorudko, I. V.</au><au>Sokolov, A. V.</au><au>Cherenkevich, S. N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gallocyanine as a Fluorogen for the Identification of NADPH-Dependent Production of Superoxide Anion Radical by Blood Cells</atitle><jtitle>Russian journal of bioorganic chemistry</jtitle><stitle>Russ J Bioorg Chem</stitle><date>2021</date><risdate>2021</risdate><volume>47</volume><issue>1</issue><spage>299</spage><epage>306</epage><pages>299-306</pages><issn>1068-1620</issn><eissn>1608-330X</eissn><abstract>—
The interaction of the oxazine dye gallocyanine with reactive oxygen (
Н
2
О
2
) and halogen (HOCl) species has been studied by spectrophotometry, mass spectrometry, and spectrofluorimetry. It has been shown that gallocyanine reacts with HOCl and
(but not with Н
2
О
2
) to form fluorescent products. It has been found using the inhibition assay that, both in the xanthine/xanthine oxidase system and in activated human blood neutrophils, the main contribution to the conversion of gallocyanine to a fluorophore comes from its reaction with
The results obtained suggest that gallocyanine can act as a fluorogenic chemosensor and be used for estimating the activation of neutrophils and the NADPH-dependent production of superoxide anion radical by neutrophils and other blood cells, as well as for testing antioxidant drugs designed with the aim to correct diseases associated with oxidative stress.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S1068162021010179</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1068-1620 |
| ispartof | Russian journal of bioorganic chemistry, 2021, Vol.47 (1), p.299-306 |
| issn | 1068-1620 1608-330X |
| language | eng |
| recordid | cdi_proquest_journals_2503296995 |
| source | Springer Nature |
| subjects | Anions Antioxidants Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Blood cells Chemical sensors Chemoreceptors Fluorescence Life Sciences Mass spectrometry Neutrophils Organic Chemistry Oxidation Spectrofluorimetry Spectrophotometry Xanthine oxidase |
| title | Gallocyanine as a Fluorogen for the Identification of NADPH-Dependent Production of Superoxide Anion Radical by Blood Cells |
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