Novel electrochemical biosensing platform for microRNA detection based on G-quadruplex formation in nanochannels

[Display omitted] •A label-free electrochemical biosensing platform for microRNA was established.•Formation of G-quadruplex/hemin complex in nanochannels was performed.•A ultralow detection limit of 0.5 aM was obtained for MiR-21 detection. Ultrasensitive detection of microRNA is crucial important i...

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Published in:Sensors and actuators. B, Chemical Chemical, 2021-01, Vol.327, p.128898, Article 128898
Main Authors: Zhao, Fei, Zhang, Hongfang, Zheng, Jianbin
Format: Article
Language:English
Subjects:
Carbon fibers
Deoxyribonucleic acid
DNA
Electrochemical analysis
Electrochemical biosensing platform
G-quadruplex
Methylene blue
microRNA
MicroRNAs
Nanochannels
Nanofibers
Ribonucleic acid
RNA
Steric hindrance
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Summary:[Display omitted] •A label-free electrochemical biosensing platform for microRNA was established.•Formation of G-quadruplex/hemin complex in nanochannels was performed.•A ultralow detection limit of 0.5 aM was obtained for MiR-21 detection. Ultrasensitive detection of microRNA is crucial important in the diagnosis of related diseases. By immobilizing the capture probes in nanochannels, we developed a label-free electrochemical biosensing platform based on guanine-quadruplex (G-quadruplex) formation in nanochannels. The designed capture probe contains both the microRNA-21 (MiR-21) recognition sequence and guanine-rich sequence. The presence of the target MiR-21 would trigger the enzymatic cleaving of the RNA/DNA heteroduplexes which led some of the guanine-rich sequences escape from the nanochannels and thus reduced the formation of the G-quadruplex/hemin complex. Therefore, the quantity of methylene blue fluxed through the nanochannels changed because of the variation of the steric hindrance. A optimized carbon nanofibers modified electrode was applied to monitor the quantity difference of methylene blue. In this way, the biosensing platform realized the label-free electrochemical detection of MiR-21 with a ultralow detection limit of 0.5 aM. Meanwhile, this biosensing platform was assessed for MiR-21 detection in serum samples. The biosensing platform can be readily extended to the assay of other scarcetumor-related genes.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2020.128898