Microlens array snapshot hyperspectral microscopy system for the biomedical domain

• Published in: Applied optics (2004) 2021-03, Vol.60 (7), p.1896
• Main Authors: Yu, Changben, Yang, Jin, Song, Nan, Sun, Ci, Wang, Mingjia, Feng, Shulong
• Format: Article
• Language: English
• Subjects: Arrays; Biological effects; Biological properties; Biomedical Research; Diagnostic Imaging - instrumentation; Domains; Equipment Design; Humans; Hyperspectral imaging; Imaging, Three-Dimensional - methods; Microlenses; Microscopes; Microscopy - instrumentation; Sampling; Spatial resolution; Spectra; Spectral resolution; Spectrum Analysis; Stomach Neoplasms - pathology
• ISSN: 1559-128X; 2155-3165
• DOI: 10.1364/AO.417952

We propose a microlens array-type snapshot hyperspectral microscope system that can provide spatial spectrum sampling according to detector frame rates for the biomedical domain. The system uses a shared optical path design. One path is used to perform direct microscopic imaging with high spatial resolution, while the other is used to collect microscopic images through a microlens array; the images are then spatially cut and reimaged such that they are spaced simultaneously by the prism-grating type hyperspectral imager's dispersion. Rapid acquisition of a three-dimensional data cube measuring 28×14×180 ( × × ) can be performed at the detector's frame rate. The system has a spatial resolution of 2.5 µm and can achieve 180-channel sampling of a 100 nm spectrum in the 400-800 nm spectral range with spectral resolution of approximately 0.56 nm. Spectral imaging results from biological samples show that the microlens array-type snapshot hyperspectral microscope system may potentially be applied in real-time biological spectral imaging.