Glyphosate in Portuguese Adults – A Pilot Study

• Published in: Environmental toxicology and pharmacology 2020-11, Vol.80, p.103462, Article 103462
• Main Authors: Nova, Paulo, Calheiros, Cristina S.C., Silva, Margarida
• Format: Article
• Language: English
• Subjects: Adult; Adults; Aged; AMPA; Biocides; Biological Monitoring; Carcinogens; Chromatography; Environmental exposure; Exposure; Female; Gas chromatography; Glycine - analogs & derivatives; Glycine - urine; Glyphosate; Herbicides; Herbicides - urine; High performance liquid chromatography; Human biomonitoring; Humans; Liquid chromatography; Male; Mass spectrometry; Mass spectroscopy; Metabolites; Middle Aged; Organophosphonates - urine; Pesticides; Pilot Projects; Portugal; Quadrupoles; Risk Assessment; Scientific imaging; Spectroscopy; Urine analysis; White People; Young Adult
• ISSN: 1382-6689; 1872-7077
• DOI: 10.1016/j.etap.2020.103462

•First study that reports glyphosate and AMPA contamination among Portuguese adults.•Pesticide residues were bio monitored in urine by GC-MS-MS and HPLC-MS/MS.•Relevant pesticide residues above the ones obtained in previous studies were detected.•Future researches about contamination source(s) are imperative. Glyphosate is a broad-spectrum biocide and the active ingredient in the most widely used herbicides worldwide. Since 2015, when the International Agency for Research on Cancer classified it as a Class 2A carcinogen, global interest in this chemical spiked particularly as regards exposure of the general population. An exploratory glyphosate exposure assessment was conducted among Portuguese adults. Self-selected participants provided first morning urine which was tested for glyphosate and its metabolite aminomethylphosphonic acid (AMPA) at two distinct periods of time, by two different laboratories using gas chromatography with tandem mass spectrometry (GC-MS-MS) and high performance liquid chromatography linked to triple quadrupole mass spectrometry (HPLC-MS/MS), respectively. In the first round of testing 28% and 50% presented detectable levels of glyphosate and AMPA respectively, with median values of 0.25 and 0.16 μg/L. Systematically available internal dose values were 8.20E-06 mg/Kg (glyphosate) and 5.04-05 mg/Kg (AMPA). In the second round 73% and 97% presented detectable levels of glyphosate and AMPA respectively with median values of 0.13 and 0.10 μg/L. Systematically available internal dose values were 4.00E-06 mg/Kg (glyphosate) and 3.00E-06 mg/Kg (AMPA). Glyphosate exposure was detected among Portuguese adults, with percentages of glyphosate and AMPA contaminated urine in both rounds of testing and above values from previous studies in other European countries. Systematically available internal doses values were below EFSA’s risk assessment values (ADI or AOEL), and as such, the concentration values measured in this study are not per se a human health problem. Even though there were study limitations, it is the first assessment in Portugal and contributes to the overall knowledge map of glyphosate exposure in Europe.