High-fat diet effect on periapical lesions and hepatic enzymatic antioxidant in rats

• Published in: Life sciences (1973) 2021-01, Vol.264, p.118637, Article 118637
• Main Authors: Tibúrcio-Machado, Camilla dos Santos, Lang, Pauline Mastella, Campos, Maria Martha, Wolle, Carlos Frederico Brilhante, Barcelos, Raquel Cristine Silva, Zago, Natália Brezolin, Leite, Carlos Eduardo, Etges, Adriana, Bier, Carlos Alexandre Souza
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants; Antioxidants - metabolism; Biochemical analysis; Blood; Blood glucose; Body size; Body weight; Catalase; Catalase - metabolism; Cholesterol; Diet; Diet, High-Fat; Euthanasia; Glucose; Glucose tolerance; Glucose Tolerance Test; Glutathione; Glutathione - metabolism; High fat diet; Inflammation; Insulin; Insulin Resistance; Lipids; Lipids - blood; Liver - enzymology; Male; Molars; Oxidative Stress; Periapical lesion; Periapical periodontitis; Periodontitis; Pulp; Radiographs; Radiography; Rats; Rats, Wistar; Rodents; Sucrose
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2020.118637

To evaluate the effects of a high-fat diet (HFD) on the progression of apical periodontitis (AP), local inflammation, systemic antioxidant status, and blood lipid profile in rats. Sixteen male Wistar rats were fed a standard diet (SD) or a HFD. At the sixth experimental week, the pulp chambers of the mandibular first molars were exposed to develop AP. A glucose tolerance test was performed the week before euthanasia. At the tenth experimental week, the animals were euthanized and the livers were collected to estimate catalase (CAT) and reduced glutathione (GSH) levels. Blood was acquired for biochemical analysis. The size of AP was estimated from radiographs and described as AP size-to-body weight ratio; inflammatory grade of AP was determined by histological analysis. At the end of the experimental period, the rats fed the HFD had 30% less weight (P