Protective effect of gamma-oryzanol against manganese-induced toxicity in Drosophila melanogaster

• Published in: Environmental science and pollution research international 2021-04, Vol.28 (14), p.17519-17531
• Main Authors: Silva, Neicí Cáceres, Poetini, Márcia Rósula, Bianchini, Matheus Chimelo, Almeida, Francielli Polet, Dahle, Mustafá Munir Mustafa, Araujo, Stífani Machado, Bortolotto, Vandreza Cardoso, Musachio, Elize Aparecida Santos, Ramborger, Bruna Piaia, Novo, Diogo La Rosa, Roehrs, Rafael, Mesko, Marcia Foster, Prigol, Marina, Puntel, Robson Luiz
• Format: Article
• Language: English
• Subjects: Accumulation; Acetylcholinesterase; Animals; Antioxidants; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Cell viability; Dopamine; Drosophila melanogaster; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Flies; Fruit flies; Hydroxylase; Insects; Intoxication; Locomotor activity; Manganese; Manganese - toxicity; Mitochondria; Mortality; Neuroprotection; Oxidative Stress; Phenylpropionates; Research Article; Toxicity; Tyrosine; Tyrosine 3-monooxygenase; Waste Water Technology; Water Management; Water Pollution Control
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-020-11848-z

Manganese (Mn) is an essential element that, in excess, seems to be involved in the development of different neurodegenerative conditions. Gamma-oryzanol (Ory) was previously reported to possess antioxidant and neuroprotective properties. Thus, we conducted this study to test the hypothesis that Ory can also protect flies in an Mn intoxication model. Adult wild-type flies were fed over 10 days with Mn (5 mM) and/or Ory (25 μM). Flies treated with Mn had a decrease in locomotor activity and a higher mortality rate compared to those in controls. Mn-treated flies also had a significant increase in acetylcholinesterase (AChE) activity, in Mn accumulation and in oxidative stress markers. Moreover, flies treated with Mn exhibited a significant decrease in dopamine levels and in tyrosine hydroxylase activity, as well as in mitochondrial and cellular viability. Particularly important, Ory protected against mortality and avoided locomotor and biochemical changes associated with Mn exposure. However, Ory did not prevent the accumulation of Mn. The present results support the notion that Ory effectively attenuates detrimental changes associated with Mn exposure in Drosophila melanogaster , reinforcing its neuroprotective action/potential.