Nickel Hydroxide Nanoparticles for Application in Immunochromatographic Strip Tests of Melamine

Nickel hydroxide nanoparticles [Ni(OH)2-NPs] synthesized by a complexation-precipitation method under hydrothermal conditions were employed as a probe in immunochromatographic strip tests for the selective detection of melamine. The characterization of Ni(OH)2-NPs and their conjugation to anti-melam...

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Bibliographic Details
Published in:Sensors and materials 2021-03, Vol.33 (3), p.1027
Main Authors: Saepudin, Endang, Yuliani, Tri, Musyarofah, Neneng Rida Rifaatul, Nasution, Mochammad Arfin Fardiansyah, Gunlazuardi, Jarnuzi, Einaga, Yasuaki, Ivandini, Tribidasari Anggraningrum
Format: Article
Language:English
Subjects:
Antibodies
Conjugation
Fourier transforms
Infrared spectrophotometers
Melamine
Nanoparticles
Nickel compounds
Strip
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Summary:Nickel hydroxide nanoparticles [Ni(OH)2-NPs] synthesized by a complexation-precipitation method under hydrothermal conditions were employed as a probe in immunochromatographic strip tests for the selective detection of melamine. The characterization of Ni(OH)2-NPs and their conjugation to anti-melamine antibody (anti-MEL) were performed using a UV–visible spectrophotometer, Fourier transform infrared (FTIR) spectroscopy, and TEM. On the basis of the green line intensity, the strip test can selectively detect melamine at a minimum concentration of 25 ppm. Our results suggest that this system is promising for the quantitative detection of melamine.
ISSN:0914-4935
2435-0869
DOI:10.18494/SAM.2021.3214