Response of bEnd.3 cells to growing behavior on the graphene oxide film with 2-D grating structure by two-beam laser interference

Graphene (G) and its derivatives are important nanomaterials with potential medical applications for biosensors and implanting biomaterials. The hydrophobicity and surface microstructures of substrates have great influences on the biological and physical properties of the surface-bound cells. In thi...

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Bibliographic Details
Published in:Applied nanoscience 2021-04, Vol.11 (4), p.1141-1149
Main Authors: Yan, Jin, Cao, Liang, Wang, Lu, Xie, Chengcheng, Liu, Yan, Song, Zhengxun, Xu, Hongmei, Weng, Zhankun, Wang, Zuobin, Li, Li
Format: Article
Language:English
Subjects:
Biological properties
Biomedical materials
Biosensors
Chemistry and Materials Science
Functional groups
Graphene
Hydrophobicity
Interference
Laser beams
Lasers
Materials Science
Membrane Biology
Morphology
Nanochemistry
Nanomaterials
Nanotechnology
Nanotechnology and Microengineering
Original Article
Oxide coatings
Physical properties
Regeneration
Repair
Substrates
Surgical implants
Tissue engineering
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Summary:Graphene (G) and its derivatives are important nanomaterials with potential medical applications for biosensors and implanting biomaterials. The hydrophobicity and surface microstructures of substrates have great influences on the biological and physical properties of the surface-bound cells. In this work, we used the two-beam laser interference (TBLI) technique to prepare a two-dimensional (2-D) grating structure on the surface of graphene oxide (GO) film. We investigated the effect of GO and the GO film with the 2-D grating structure substrates on the growth behavior of rat brain microvascular endothelial (bEnd.3) cells. The results demonstrated that the cell spreading area and the number of surface-bound cells were closely related to the hydrophobicity of the substrate and the presence of oxygen-containing functional groups (OCGs). Due to the interaction of laser and GO, the GO in the interference area was transformed into reduced graphene oxide (RGO). The grating-structured GO film significantly affected the direction of cell spreading and morphology. It has a good application prospect as a scaffold in tissue engineering, and promising applications in the fields that require highly directional growth of cells, such as nerve injury repair, tendon repair and regeneration.
ISSN:2190-5509
2190-5517
DOI:10.1007/s13204-021-01701-1