SARS-CoV-2 epitope mapping on microarrays highlights strong immune-response to N protein region

Abstract A workflow for SARS-CoV-2 epitope discovery on peptide microarrays is herein reported. The process started with a proteome-wide screening of immunoreactivity based on the use of a high-density microarray followed by a refinement and validation phase on a restricted panel of probes using mic...

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Bibliographic Details
Published in:bioRxiv 2020-11
Main Authors: Musicò, Angelo, Frigerio, Roberto, Mussida, Alessandro, Barzon, Luisa, Sinigaglia, Alessandro, Riccetti, Silvia, Gobbi, Federico, Piubelli, Chiara, Bergamaschi, Greta, Chiari, Marcella, Gori, Alessandro, Cretich, Marina
Format: Article
Language:English
Subjects:
Coronaviruses
COVID-19
Epitope mapping
Immobilization
Immunoglobulin G
Immunoreactivity
N protein
Nucleocapsids
Peptide mapping
Peptides
Proteins
Proteomes
Serological tests
Severe acute respiratory syndrome coronavirus 2
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Summary:Abstract A workflow for SARS-CoV-2 epitope discovery on peptide microarrays is herein reported. The process started with a proteome-wide screening of immunoreactivity based on the use of a high-density microarray followed by a refinement and validation phase on a restricted panel of probes using microarrays with tailored peptide immobilization through a click-based strategy. Progressively larger, independent cohorts of Covid-19 positive sera were tested in the refinement processes, leading to the identification of immunodominant regions on SARS-CoV-2 Spike (S), Nucleocapsid (N) protein and Orf1ab polyprotein. A summary study testing 50 serum samples highlighted an epitope of the N protein (region 155-171) providing 92% sensitivity and 100% specificity of IgG detection in Covid-19 samples thus being a promising candidate for rapid implementation in serological tests. Competing Interest Statement The authors have declared no competing interest.
DOI:10.1101/2020.11.09.374082